MicroRNA-30a Inhibits Colorectal Cancer Metastasis Through Down-Regulation of Type I Insulin-Like Growth Factor Receptor.

Abstract

BACKGROUND:

miR-30a expression is down-regulated and regulates tumor suppressors in various cancers.

AIM:

We investigated the mechanisms underlying the biological role of miR-30a in CRC.

METHODS:

MicroRNA, mRNA, and protein expression were analyzed by quantitative real-time PCR and Western blot. The migration and invasion abilities of CRC were determined by wound healing assay, and trans-well migration and invasion. A luciferase reporter assay was used to confirm the targets of miR-30a.

RESULTS:

miR-30a expression was significantly down-regulated in CRC tissues and in CRC tissue with lymph node metastasis compared to CRC tissue without metastasis. Overexpression of miR-30a suppressed migration and invasion through insulin-like growth factor 1 receptor (IGF1R) in CRC cells. miR-30a suppresses IGF1R protein expression and inhibits β-catenin or p-AKT and increases E-cadherin expression. The IGF1R expression level is also up-regulated in CRC tumors and inversely correlated with miR-30a in CRC specimens.

CONCLUSIONS:

miR-30a functions as a tumor-suppressive miRNA, which may provide a therapeutic strategy for metastasis of CRC.

• Authors: Y C Liu, Y R Park, S L Kim, S T Lee, S W Kim
• Journal: Digestive diseases and sciences (Dig Dis Sci) Vol. 62 Issue 11 Pg. 3040-3049 (11 2017) ISSN: 1573-2568 [Electronic] United States
• PMID: 28932920 (Publication Type: Journal Article)

Chemical References

• 3' Untranslated Regions
• Antigens, CD
• CDH1 protein, human
• CTNNB1 protein, human
• Cadherins
• IGF1R protein, human
• MIRN30b microRNA, human
• MicroRNAs
• RNA, Messenger
• Receptors, Somatomedin
• beta Catenin
• Receptor, IGF Type 1
• Proto-Oncogene Proteins c-akt

Topics

• 3' Untranslated Regions
• Aged
• Antigens, CD
• Binding Sites
• Cadherins (metabolism)
• Cell Line, Tumor
• Cell Movement
• Colorectal Neoplasms (genetics, metabolism, pathology)
• Down-Regulation
• Female
• Gene Expression Regulation, Neoplastic
• HEK293 Cells
• Humans
• Male
• MicroRNAs (genetics, metabolism)
• Middle Aged
• Neoplasm Metastasis
• Phosphorylation
• Proto-Oncogene Proteins c-akt (metabolism)
• RNA, Messenger (genetics, metabolism)
• Receptor, IGF Type 1
• Receptors, Somatomedin (genetics, metabolism)
• Signal Transduction
• Transfection
• beta Catenin (metabolism)