Deletion of NADH oxidase in Listeria monocytogenes promotes the bacterial infection of brain.

Abstract

NADH oxidase (NOX) plays important roles in respiration and reactive oxygen species (ROS) generation in cells. In this study, we explored the function of NOX in Listeria monocytogenes by gene deletion. From our results, nox mutant strain (∆nox) had lower H2O2 level and showed no significant alteration in bacteria growth activity. But it had enhanced invasiveness during the invasion of glial cells and mice brain compared to wildtype strain. Furthermore, several virulence genes involved in invasion, such as inlA, inlB, vip and sigB, were upregulated in ∆nox, and the alterations could be restored by complementation. To explore if nox was involved in the interaction of pathogen and host, we examined the generation of host ROS including superoxide and H2O2 during infection, and found ∆nox invasion leading to less superoxide and H2O2 generation. Besides, the upregulation of pro-inflammatory factors in glial cells was restrained when invaded by ∆nox compared to wildtype and complementary strain. In conclusion, our study evaluated the function of nox in L. monocytogenes and indicated that nox could regulate the invasion of L. monocytogenes by regulating virulence genes expression and the interaction of host-and- pathogens.

• Authors: Sen Li, Wenwen Yu, Xiao Guan, Zhen Luo, Guowei Chen, Wukang Liu, Jingchen Zhang
• Journal: Free radical biology & medicine (Free Radic Biol Med) Vol. 112 Pg. 608-615 (11 2017) ISSN: 1873-4596 [Electronic] United States
• PMID: 28916475 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2017 Elsevier Inc. All rights reserved.

Chemical References

• Bacterial Proteins
• Membrane Proteins
• SigB protein, Listeria monocytogenes
• Sigma Factor
• Vip protein, Listeria monocytogenes
• Virulence Factors
• inlB protein, Listeria monocytogenes
• Superoxides
• internalin protein, Bacteria
• Hydrogen Peroxide
• NADPH Oxidases

Topics

• Animals
• Bacterial Proteins (genetics, metabolism)
• Base Sequence
• Brain (metabolism, microbiology, pathology)
• Gene Expression Regulation, Bacterial
• Genetic Complementation Test
• Host-Pathogen Interactions
• Hydrogen Peroxide (metabolism)
• Listeria monocytogenes (genetics, growth & development, pathogenicity)
• Listeriosis (genetics, metabolism, microbiology, pathology)
• Membrane Proteins (genetics, metabolism)
• Mice
• NADPH Oxidases (deficiency, genetics)
• Neuroglia (metabolism, microbiology, pathology)
• Sequence Deletion
• Sigma Factor (genetics, metabolism)
• Signal Transduction
• Superoxides (metabolism)
• Virulence Factors (genetics, metabolism)