Lycopene rich extract from red guava (Psidium guajava L.) displays anti-inflammatory and antioxidant profile by reducing suggestive hallmarks of acute inflammatory response in mice.

Abstract

This study investigated the anti-inflammatory activity of the extract (LEG) and purified (LPG) lycopene from guava (Psidium guajava L.), as well as some mechanisms possibly involved in this effect. The anti-inflammatory activity was initially assessed using paw edema induced by Carrageenan, Dextran, Compound 48/80, Histamine and Prostaglandin E2 in Swiss mice. A peritonitis model was used to evaluate neutrophil migration, the activity of myeloperoxidase (MPO) and reduced glutathione (GSH) concentration; while the effect on the expression of iNOS, COX-2 and NF-κB, was assessed by immunohistochemistry analysis. Results showed that oral and intraperitoneal administration of LEG and LPG inhibited inflammation caused by carrageenan. LPG (12.5mg/kg p.o.) significantly inhibited the edema formation induced by different phlogistic agents and immunostaining for iNOS, COX-2 and NF-κB. Leukocytes migration in paw tissue and peritoneal cavity was reduced, as well as MPO concentration, whereas GSH levels increased. Thus, lycopene-rich extract from red guava has beneficial effect on acute inflammation, offering protection against the consequences of oxidative stress by downregulating inflammatory mediators and inhibiting gene expression involved in inflammation.

• Authors: Andreanne G Vasconcelos, Adriany das G N Amorim, Raimunda C Dos Santos, Jessica Maria T Souza, Luan Kelves M de Souza, Thiago de S L Araújo, Lucas Antonio D Nicolau, Lucas de Lima Carvalho, Pedro Everson A de Aquino, Conceição da Silva Martins, Cristina D Ropke, Pedro Marcos G Soares, Selma Aparecida S Kuckelhaus, Jand-Venes R Medeiros, José Roberto de S A Leite
• Journal: Food research international (Ottawa, Ont.) (Food Res Int) Vol. 99 Issue Pt 2 Pg. 959-968 (09 2017) ISSN: 1873-7145 [Electronic] Canada
• PMID: 28847433 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2017 Elsevier Ltd. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Inflammation Mediators
• NF-kappa B
• Plant Extracts
• Peroxidase
• Nitric Oxide Synthase Type II
• Nos2 protein, mouse
• Ptgs2 protein, mouse
• Cyclooxygenase 2
• Glutathione

Topics

• Animals
• Anti-Inflammatory Agents (isolation & purification, pharmacology)
• Antioxidants (isolation & purification, pharmacology)
• Cyclooxygenase 2 (metabolism)
• Disease Models, Animal
• Edema (immunology, metabolism, prevention & control)
• Female
• Fruit
• Glutathione (metabolism)
• Inflammation (immunology, metabolism, prevention & control)
• Inflammation Mediators (metabolism)
• Leukocytes (drug effects, immunology, metabolism)
• Male
• Mice
• NF-kappa B (metabolism)
• Neutrophil Infiltration (drug effects)
• Nitric Oxide Synthase Type II (metabolism)
• Oxidative Stress (drug effects)
• Peritonitis (immunology, metabolism, prevention & control)
• Peroxidase (metabolism)
• Plant Extracts (isolation & purification, pharmacology)
• Psidium (chemistry)