Effects of meglumine cyclic adenylate pretreatment on systemic inflammatory response syndrome induced by lipopolysaccharide in rats.

Abstract

Studies showed that the use of cyclic adenosine monophosphate (cAMP) substitutes or intracellular cAMP activators increased intracellular cAMP level, causing anti-inflammatory effects. This study was to investigate the effects of pretreatment with meglumine cyclic adenylate (MCA), a compound of meglumine and cAMP, on systemic inflammation induced by lipopolysaccharide (LPS) in rats. Eighteen adult male Sprague-Dawley rats were randomly divided into 3 groups (n=6 each): control group (NS group), LPS group (LPS group) and LPS with MCA pretreatment group (MCA group). Systemic inflammation was induced with LPS 10 mg/kg injected via the femoral vein in LPS and MCA groups. In MCA group, MCA 2 mg/kg was injected via the femoral vein 20 min before LPS injection, and the equal volume of normal saline was given in NS and LPS groups at the same time. Three hours after LPS injection, the blood samples were taken from the abdominal aorta for determination of plasma concentrations of TNF-α, IL-1, IL-6, IL-10, cAMP by ELISA and NF-κBp65 expression by Western blotting. The experimental results showed that inflammatory and antiinflammatory indices were increased in LPS group compared to NS group; inflammatory indices were declined and anti-inflammatory indices were increased in MCA group relative to LPS group. Our study suggested that MCA pretreatment may attenuate LPS-induced systemic inflammation.

• Authors: Wei Liu, Jing-Li Chen, Henry Liu, Hong Yan
• Journal: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (J Huazhong Univ Sci Technolog Med Sci) Vol. 37 Issue 3 Pg. 332-336 (Jun 2017) ISSN: 1993-1352 [Electronic] China
• PMID: 28585140 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Biomarkers
• Interleukin-1
• Interleukin-6
• Lipopolysaccharides
• Rela protein, rat
• Transcription Factor RelA
• Tumor Necrosis Factor-alpha
• meglumine cyclic adenylate
• Interleukin-10
• Meglumine
• Cyclic AMP

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Biomarkers (blood)
• Cyclic AMP (analogs & derivatives, blood, immunology, pharmacology)
• Injections, Intravenous
• Interleukin-1 (blood, immunology)
• Interleukin-10 (blood, immunology)
• Interleukin-6 (blood, immunology)
• Lipopolysaccharides
• Male
• Meglumine (analogs & derivatives, pharmacology)
• Rats
• Rats, Sprague-Dawley
• Systemic Inflammatory Response Syndrome (blood, chemically induced, immunology, prevention & control)
• Transcription Factor RelA (blood, immunology)
• Tumor Necrosis Factor-alpha (blood, immunology)