Serum Level of Antibodies (IgG, IgM) Against Benzo[a]pyrene-7,8-diol-9,10-epoxide-DNA Adducts in Children Dermatologically Exposed to Coal Tar.

Abstract

Crude coal tar (CCT) contains polycyclic aromatic hydrocarbons (PAHs). Benzo[a]pyrene (BaP) is metabolized into a highly reactive metabolite benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE) that is able to bind to DNA and creates BPDE-DNA adducts. Adducted DNA becomes immunogenic and induces immune response by production of antibodies against BPDE-DNA adducts (Ab-BPDE-DNA). Circulating Ab-BPDE-DNA was proposed as potential biomarker of genotoxic exposure to BaP (PAHs). Goeckerman therapy (GT) of psoriasis uses dermal application of CCT ointment (PAHs). In presented study (children with psoriasis treated by GT; n = 19) the therapy significantly increased the level of Ab-BPDE-DNA (EI = 0.29/0.19-0.34 vs. 0.31/0.25-0.40; median/lower-upper quartile; p < 0.01). The results support the idea of Ab-BPDE-DNA level as a possible tentative indicator of exposure, effects and susceptibility of the organism to the exposure of BaP (PAHs).

• Authors: Pavel Borský, Ctirad Andrýs, Jan Krejsek, Květoslava Hamáková, Jan Kremláček, Andrea Málková, Lenka Bartošová, Zdeněk Fiala, Vladimír Palička, Lenka Borská
• Journal: Acta medica (Hradec Kralove) (Acta Medica (Hradec Kralove)) 2017 Vol. 60 Issue 1 Pg. 27-31 ISSN: 1211-4286 [Print] Czech Republic
• PMID: 28467299 (Publication Type: Journal Article)

Chemical References

• DNA Adducts
• Keratolytic Agents
• 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
• Coal Tar

Topics

• 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (analysis)
• Child
• Child, Preschool
• Coal Tar (adverse effects, therapeutic use)
• DNA Adducts (blood, drug effects)
• DNA Damage (drug effects)
• Humans
• Keratolytic Agents (administration & dosage, therapeutic use)
• Psoriasis (drug therapy)