Expression of lactate dehydrogenase C correlates with poor prognosis in renal cell carcinoma.

Abstract

Lactate dehydrogenase C is an isoenzyme of lactate dehydrogenase and a member of the cancer-testis antigens family. In this study, we aimed to investigate the expression and functional role of lactate dehydrogenase C and its basic mechanisms in renal cell carcinoma. First, a total of 133 cases of renal cell carcinoma samples were analysed in a tissue microarray, and Kaplan-Meier survival curve analyses were performed to investigate the correlation between lactate dehydrogenase C expression and renal cell carcinoma progression. Lactate dehydrogenase C protein levels and messenger RNA levels were significantly upregulated in renal cell carcinoma tissues, and the patients with positive lactate dehydrogenase C expression had a shorter progression-free survival, indicating the oncogenic role of lactate dehydrogenase C in renal cell carcinoma. In addition, further cytological experiments demonstrated that lactate dehydrogenase C could prompt renal cell carcinoma cells to produce lactate, and increase metastatic and invasive potential of renal cell carcinoma cells. Furthermore, lactate dehydrogenase C could induce the epithelial-mesenchymal transition process and matrix metalloproteinase-9 expression. In summary, these findings showed lactate dehydrogenase C was associated with poor prognosis in renal cell carcinoma and played a pivotal role in the migration and invasion of renal cell carcinoma cells. Lactate dehydrogenase C may act as a novel biomarker for renal cell carcinoma progression and a potential therapeutic target for the treatment of renal cell carcinoma.

• Authors: Yibo Hua, Chao Liang, Jundong Zhu, Chenkui Miao, Yajie Yu, Aimin Xu, Jianzhong Zhang, Pu Li, Shuang Li, Meiling Bao, Jie Yang, Chao Qin, Zengjun Wang
• Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (Tumour Biol) Vol. 39 Issue 3 Pg. 1010428317695968 (Mar 2017) ISSN: 1423-0380 [Electronic] Netherlands
• PMID: 28351304 (Publication Type: Journal Article)

Chemical References

• Antigens, Neoplasm
• Biomarkers, Tumor
• Isoenzymes
• L-Lactate Dehydrogenase
• lactate dehydrogenase C4
• MMP9 protein, human
• Matrix Metalloproteinase 9

Topics

• Adult
• Antigens, Neoplasm (biosynthesis, genetics)
• Biomarkers, Tumor (biosynthesis, genetics)
• Carcinoma, Renal Cell (genetics, pathology)
• Disease-Free Survival
• Epithelial-Mesenchymal Transition (genetics)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Isoenzymes (biosynthesis, genetics)
• L-Lactate Dehydrogenase (biosynthesis, genetics)
• Male
• Matrix Metalloproteinase 9 (biosynthesis)
• Middle Aged
• Neoplasm Metastasis
• Prognosis