Effect of butyrate and Lactobacillus GG on a butyrate receptor and transporter during Campylobacter jejuni exposure.

Abstract

Campylobacter jejuni frequently infects humans causing many gastrointestinal symptoms, fever, fatigue and several long-term debilitating diseases. Current treatment for campylobacteriosis includes rehydration and in some cases, antibiotic therapy. Probiotics are used to treat several gastrointestinal diseases. Butyrate is a short-chain fatty acid known to promote intestinal health. Interaction of butyrate with its respective receptor (HCAR2) and transporter (SLC5A8), both expressed in the intestine, is associated with water and electrolyte absorption as well as providing defense against colon cancer and inflammation. Alterations in gut microbiota influence the presence of HCAR2 and SLC5A8 in the intestine. We hypothesized that adherence and/or invasion of C. jejuni and alterations in HCAR2 and SLC5A8 expression would be minimized with butyrate or Lactobacillus GG (LGG) pretreatment of Caco-2 cells. We found that both C. jejuni adhesion but not invasion was reduced with butyrate pretreatment. While LGG pretreatment did not prevent C. jejuni adhesion, it did result in reduced invasion which was associated with altered cell supernate pH. Both butyrate and LGG protected HCAR2 and SLC5A8 protein expression following C. jejuni infection. These results suggest that the first stages of C. jejuni infection of Caco-2 cells may be minimized by LGG and butyrate pretreatment.

• Authors: Gail A M Cresci, Paul C Mayor, Stuart A Thompson
• Journal: FEMS microbiology letters (FEMS Microbiol Lett) Vol. 364 Issue 6 (03 01 2017) ISSN: 1574-6968 [Electronic] England
• PMID: 28333199 (Publication Type: Journal Article)
• Copyright: © FEMS 2017. All rights reserved. For permissions, please e-mail: [email protected].

Chemical References

• Butyrates
• Carrier Proteins
• HCAR2 protein, human
• Monocarboxylic Acid Transporters
• Receptors, G-Protein-Coupled
• Receptors, Nicotinic
• SLC5A8 protein, human

Topics

• Bacterial Adhesion (drug effects)
• Butyrates (metabolism, pharmacology)
• Caco-2 Cells
• Campylobacter Infections (genetics, metabolism, microbiology)
• Campylobacter jejuni (physiology)
• Carrier Proteins (metabolism)
• Cells, Cultured
• Gene Expression
• Humans
• Hydrogen-Ion Concentration
• Lacticaseibacillus rhamnosus (drug effects, metabolism)
• Microbial Interactions
• Monocarboxylic Acid Transporters (genetics, metabolism)
• Receptors, G-Protein-Coupled (genetics, metabolism)
• Receptors, Nicotinic (genetics, metabolism)