Abstract | PURPOSE: MATERIALS AND METHODS: In this study, a proteomics assay was used to detect the expression of proteins after HuR downregulation, and a luciferase assay was used to detect the potential presence of a HuR binding site on the 3'-untranslated region (3'-UTR) of interleukin 18 (IL-18). In addition, colony formation assay, MTT, EdU incorporation assay, Western blot, flow cytometry, immunohistochemistry, transwell invasion assay, and wound healing assay were used. RESULTS: In the present study, we found that the expression of both HuR protein and mRNA levels were higher in tumor tissues than in the adjacent tissues. HuR downregulation significantly suppressed cell proliferation. In addition, the metastasis of esophageal cancer cells was inhibited, while the expression of E-cadherin was increased and the expression of matrix metalloproteinase ( MMP) 2, MMP9, and vimentin was decreased after HuR knockdown. Moreover, silencing of HuR disturbed the cell cycle of ESCC cells mainly by inducing G1 arrest. Furthermore, proteomics analysis showed that downregulation of HuR in TE-1 cells resulted in 100 upregulated and 122 downregulated proteins, including IL-18 as a significantly upregulated protein. The expression of IL-18 was inversely regulated by HuR. IL-18 expression was decreased in ESCC tissues, and exogenous IL-18 significantly inhibited the proliferation and metastasis of ESCC cells. The 3'-UTR of IL-18 harbored a HuR binding site, as shown by an in vitro luciferase assay. CONCLUSION: HuR plays an important role in the progression of esophageal carcinoma by targeting IL-18, which may be a potential therapeutic target for the treatment of ESCC.
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Authors | Xiaohui Xu, Cheng Song, Zhihua Chen, Chenxiao Yu, Yi Wang, Yiting Tang, Judong Luo |
Journal | Cancer research and treatment
(Cancer Res Treat)
Vol. 50
Issue 1
Pg. 71-87
(Jan 2018)
ISSN: 2005-9256 [Electronic] Korea (South) |
PMID | 28231690
(Publication Type: Journal Article)
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Chemical References |
- ELAV-Like Protein 1
- ELAVL1 protein, human
- IL18 protein, human
- Interleukin-18
- RNA, Messenger
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Topics |
- Cell Proliferation
- Down-Regulation
- ELAV-Like Protein 1
(biosynthesis, genetics, metabolism)
- Esophageal Neoplasms
(genetics, metabolism, pathology)
- Humans
- Immunohistochemistry
- Interleukin-18
(genetics, metabolism)
- RNA, Messenger
(biosynthesis)
- Transfection
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