HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Chemical Isotope Labeling LC-MS for Monitoring Disease Progression and Treatment in Animal Models: Plasma Metabolomics Study of Osteoarthritis Rat Model.

Abstract
We report a chemical isotope labeling (CIL) liquid chromatography mass spectrometry (LC-MS) method generally applicable for tracking metabolomic changes from samples collected in an animal model for studying disease development and treatment. A rat model of surgically induced osteoarthritis (OA) was used as an example to illustrate the workflow and technical performance. Experimental duplicate analyses of 234 plasma samples were carried out using dansylation labeling LC-MS targeting the amine/phenol submetabolome. These samples composed of 39 groups (6 rats per group) were collected at multiple time points with sham operation, OA control group, and OA rats with treatment, separately, using glucosamine/Celecoxib and three traditional Chinese medicines (Epimedii folium, Chuanxiong Rhizoma and Bushen-Huoxue). In total, 3893 metabolites could be detected and 2923 of them were consistently detected in more than 50% of the runs. This high-coverage submetabolome dataset could be used to track OA progression and treatment. Many differentiating metabolites were found and 11 metabolites including 2-aminoadipic acid, saccharopine and GABA were selected as potential biomarkers of OA progression and OA treatment. This study illustrates that CIL LC-MS is a very useful technique for monitoring incremental metabolomic changes with high coverage and accuracy for studying disease progression and treatment in animal models.
AuthorsDeying Chen, Xiaoling Su, Nan Wang, Yunong Li, Hua Yin, Liang Li, Lanjuan Li
JournalScientific reports (Sci Rep) Vol. 7 Pg. 40543 (01 16 2017) ISSN: 2045-2322 [Electronic] England
PMID28091618 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Biomarkers
  • Lysine
Topics
  • Animals
  • Biomarkers (metabolism)
  • Chromatography, Liquid (methods)
  • Discriminant Analysis
  • Disease Models, Animal
  • Disease Progression
  • Isotope Labeling (methods)
  • Joints (pathology)
  • Least-Squares Analysis
  • Lysine (metabolism)
  • Metabolome
  • Metabolomics
  • Osteoarthritis (blood, metabolism, pathology)
  • Principal Component Analysis
  • Rats, Sprague-Dawley
  • Tandem Mass Spectrometry (methods)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: