Abstract | OBJECTIVES: Listeria monocytogenes is a facultative anaerobic foodborne pathogen that can traverse the blood-brain barrier and cause brain infection. L. monocytogenes infection induces host cell apoptosis in several cell types. In this study, we investigated the apoptosis of human glioma cell line U251 invaded by L. monocytogenes and evaluated the function of bacterial reactive oxygen species (ROS) during infection. METHODS: RESULTS: DPI and NAC efficiently decreased ROS level in L. monocytogenes without affecting bacterial growth. Moreover, the apoptosis of glial cells was enhanced upon invasion of DPI- and NAC-pretreated L. monocytogenes. DISCUSSION: Results indicate that the apoptosis of glial cells can be induced by L. monocytogenes, and that the inhibition of bacterial ROS increases the apoptosis of host cells.
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Authors | Sen Li, Yixuan Li, Guowei Chen, Jingchen Zhang, Fei Xu, Man Wu |
Journal | Redox report : communications in free radical research
(Redox Rep)
Vol. 22
Issue 4
Pg. 190-196
(Jul 2017)
ISSN: 1743-2928 [Electronic] England |
PMID | 27120397
(Publication Type: Journal Article)
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Chemical References |
- Onium Compounds
- Reactive Oxygen Species
- diphenyleneiodonium
- Acetylcysteine
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Topics |
- Acetylcysteine
(pharmacology)
- Apoptosis
(drug effects, genetics, physiology)
- Cell Line, Tumor
- Humans
- Listeria monocytogenes
(metabolism, physiology)
- Neuroglia
(cytology, metabolism)
- Onium Compounds
(pharmacology)
- Reactive Oxygen Species
(metabolism)
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