Clinicopathological Analysis and Multipronged Quantitative Proteomics Reveal Oxidative Stress and Cytoskeletal Proteins as Possible Markers for Severe Vivax Malaria.

Abstract

In Plasmodium vivax malaria, mechanisms that trigger transition from uncomplicated to fatal severe infections are obscure. In this multi-disciplinary study we have performed a comprehensive analysis of clinicopathological parameters and serum proteome profiles of vivax malaria patients with different severity levels of infection to investigate pathogenesis of severe malaria and identify surrogate markers of severity. Clinicopathological analysis and proteomics profiling has provided evidences for the modulation of diverse physiological pathways including oxidative stress, cytoskeletal regulation, lipid metabolism and complement cascades in severe malaria. Strikingly, unlike severe falciparum malaria the blood coagulation cascade was not found to be affected adversely in acute P. vivax infection. To the best of our knowledge, this is the first comprehensive proteomics study, which identified some possible cues for severe P. vivax infection. Our results suggest that Superoxide dismutase, Vitronectin, Titin, Apolipoprotein E, Serum amyloid A, and Haptoglobin are potential predictive markers for malaria severity.

• Authors: Sandipan Ray, Sandip K Patel, Apoorva Venkatesh, Amruta Bhave, Vipin Kumar, Vaidhvi Singh, Gangadhar Chatterjee, Veenita G Shah, Sarthak Sharma, Durairaj Renu, Naziya Nafis, Prajakta Gandhe, Nithya Gogtay, Urmila Thatte, Kunal Sehgal, Sumit Verma, Avik Karak, Dibbendhu Khanra, Arunansu Talukdar, Sanjay K Kochar, Vijeth S B, Dhanpat K Kochar, Dharmendra Rojh, Santosh G Varma, Mayuri N Gandhi, Rapole Srikanth, Swati Patankar, Sanjeeva Srivastava
• Journal: Scientific reports (Sci Rep) Vol. 6 Pg. 24557 (Apr 19 2016) ISSN: 2045-2322 [Electronic] England
• PMID: 27090372 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Apolipoproteins E
• Biomarkers
• Connectin
• Cytoskeletal Proteins
• Haptoglobins
• Serum Amyloid A Protein
• Vitronectin
• Superoxide Dismutase

Topics

• Adult
• Apolipoproteins E (blood)
• Biomarkers (blood)
• Connectin (blood)
• Cytoskeletal Proteins (blood)
• Female
• Haptoglobins (metabolism)
• Humans
• Malaria, Vivax (blood, parasitology)
• Oxidative Stress
• Plasmodium vivax (pathogenicity)
• Proteomics
• Serum Amyloid A Protein (metabolism)
• Superoxide Dismutase (blood)
• Vitronectin (blood)