Abstract | BACKGROUND/AIMS:
Fucoxanthin, a carotenoid isolated from brown seaweeds, induces suicidal death or apoptosis of tumor cells and is thus considered for the treatment or prevention of malignancy. In analogy to apoptosis of nucleated cell, erythrocytes may enter eryptosis, the suicidal death characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface. Triggers of eryptosis include Ca2+ entry with increase of cytosolic Ca2+ activity ([Ca2+]i), oxidative stress and activation of p38 kinase or protein kinase C. The present study explored, whether and how fucoxanthin induces eryptosis. METHODS: RESULTS: A 48 hours exposure of human erythrocytes to fucoxanthin significantly increased the percentage of annexin-V-binding cells (≥ 50 µM), significantly decreased average forward scatter (≥ 25 µM), significantly increased hemolysis (≥ 25 µM), significantly increased Fluo3-fluorescence (≥ 50 µM), significantly increased lipid peroxidation, but did not significantly modify DCFDA fluorescence. The effect of fucoxanthin on annexin-V-binding was significantly blunted, but not abolished by removal of extracellular Ca2+, and was insensitive to p38 kinase inhibitor skepinone (2 µM) and to protein kinase C inhibitor calphostin (100 nM). CONCLUSION:
Fucoxanthin triggers cell shrinkage and phospholipid scrambling of the erythrocyte cell membrane, an effect in part due to stimulation of Ca2+ entry.
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Authors | Marilena Briglia, Salvatrice Calabró, Elena Signoretto, Kousi Alzoubi, Stefan Laufer, Caterina Faggio, Florian Lang |
Journal | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
(Cell Physiol Biochem)
Vol. 37
Issue 6
Pg. 2464-75
( 2015)
ISSN: 1421-9778 [Electronic] Germany |
PMID | 26666406
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2015 The Author(s) Published by S. Karger AG, Basel. |
Chemical References |
- Xanthophylls
- fucoxanthin
- p38 Mitogen-Activated Protein Kinases
- Calcium
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Topics |
- Calcium
(metabolism)
- Cell Death
(drug effects)
- Enzyme Activation
- Erythrocytes
(drug effects, metabolism)
- Humans
- Ion Transport
- Oxidative Stress
- Xanthophylls
(pharmacology)
- p38 Mitogen-Activated Protein Kinases
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