Reveromycin A Administration Prevents Alveolar Bone Loss in Osteoprotegerin Knockout Mice with Periodontal Disease.

Abstract

Chronic periodontal disease is characterized by alveolar bone loss and inflammatory changes. Reveromycin A (RMA) was recently developed and is a unique agent for inhibiting osteoclast activity. This study analysed the effects of RMA in an experimental mouse model of periodontitis involving osteoprotegerin (OPG)-knockout mice, specifically, whether it could control osteoclasts and reduce inflammation in periodontal tissue. We examined wild-type (WT) and OPG knockout mice (OPG KO) ligated with wire around contact points on the left first and second molars. RMA was administered twice a day to half of the mice. Using micro-computed tomography, we measured the volume of alveolar bone loss between the first and second molars, and also performed histological analysis. The OPG KO RMA+ group had significantly decreased osteoclast counts, alveolar bone loss, attachment loss, and inflammatory cytokine expression 8 weeks after ligation. Thus, RMA may reduce alveolar bone loss and inflamed periodontal tissues in patients with periodontitis.

• Authors: Manami Mizuno, Ken Miyazawa, Masako Tabuchi, Miyuki Tanaka, Mamoru Yoshizako, Chisato Minamoto, Yasuyoshi Torii, Yusuke Tamaoka, Makoto Kawatani, Hiroyuki Osada, Hatsuhiko Maeda, Shigemi Goto
• Journal: Scientific reports (Sci Rep) Vol. 5 Pg. 16510 (Nov 12 2015) ISSN: 2045-2322 [Electronic] England
• PMID: 26561427 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Inflammation Mediators
• Osteoprotegerin
• Pyrans
• Spiro Compounds
• reveromycin A

Topics

• Alveolar Bone Loss (diagnosis, drug therapy, etiology, pathology)
• Animals
• Cytokines (metabolism)
• Disease Models, Animal
• Inflammation Mediators (metabolism)
• Male
• Mice
• Mice, Knockout
• Osteoclasts (drug effects, metabolism)
• Osteoprotegerin (deficiency)
• Periodontal Diseases (complications, diagnosis, genetics, metabolism)
• Pyrans (pharmacology)
• Spiro Compounds (pharmacology)
• X-Ray Microtomography