Chemopreventive effect of oleuropein in colitis-associated colorectal cancer in c57bl/6 mice.

Abstract

SCOPE:

The main phenolic secoiridoid oleuropein and active constituent from olive tree (Olea europaea, Oleaceae), has demonstrated anti-inflammatory properties in intestinal inflammation and anti-tumoral effects in different cancer cells. In this study, we evaluated the chemoprevention of oleuropein in a model of azoxymethane (AOM)/Dextran sulfate sodium (DSS)-induced colorectal cancer (CRC) in C57BL/6 mice and the modulatory effect on the Th17 response in DSS acute colitis.

METHODS AND RESULTS:

Oleuropein protected from AOM/DSS-induced CRC by improving clinical symptoms, disease activity index score as well as suppressed the growth and multiplicity of colonic tumors. Treatment with oleuropein reduced intestinal IL-6, IFN-γ, TNF-α, and IL-17A concentration, and decreased cyclooxygenase-2, Bax and proliferating cell nuclear antigen protein expression. Western blot analysis also showed a markedly downregulation of CRC-related pathways as nuclear factor-κB (NF-κB), Wnt/β-catenin, phosphatidylinositol-3-kinase (P3IK)/Akt, and signal transducer and activators of transcription (STAT)3. In DSS acute model, oleuropein inhibited Th17 response, by decreasing CD4(+) Rorγt(+) IL-17(+) IFN-γ(+) T-cell subsets in the lamina propria, as well as IL-17A and IFN-γ expression.

CONCLUSION:

Oleuropein as a dietary supplementation could be a promising protective agent against colitis-associated CRC.

• Authors: Elisa Giner, M Carmen Recio, José Luis Ríos, José Miguel Cerdá-Nicolás, Rosa María Giner
• Journal: Molecular nutrition & food research (Mol Nutr Food Res) Vol. 60 Issue 2 Pg. 242-55 (Feb 2016) ISSN: 1613-4133 [Electronic] Germany
• PMID: 26502315 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Anticarcinogenic Agents
• Cytokines
• Iridoid Glucosides
• Iridoids
• oleuropein
• Dextran Sulfate
• Azoxymethane

Topics

• Animals
• Anticarcinogenic Agents (pharmacology)
• Azoxymethane (toxicity)
• Cell Proliferation (drug effects)
• Colitis (chemically induced, complications, drug therapy)
• Colon (drug effects, metabolism, pathology)
• Colorectal Neoplasms (etiology, pathology, prevention & control)
• Cytokines (metabolism)
• Dextran Sulfate (toxicity)
• Female
• Iridoid Glucosides
• Iridoids (pharmacology)
• Mice, Inbred C57BL
• Neoplasms, Experimental (prevention & control)
• Th17 Cells (drug effects)