Abstract | BACKGROUND: METHODS: We induced renal I/R injury by clamping the left renal artery for 45 min followed by reperfusion, along with a contralateral nephrectomy. We randomly assigned 30 rats to 3 groups (n = 10): sham-operated, vehicle-treated I/R, and osthole-treated I/R. We treated rats intra-peritoneally with osthole (40 mg/kg) or vehicle (40 mg/kg) 45 min before renal ischemia. We harvested serum and kidneys at 24 h after reperfusion. Renal function and histological changes were assessed. The expression of tumor necrosis factor-alpha (TNF-α), interleukin-8 (IL-8), and interleukin-6 (IL-6) in renal tissue and serum were examined by means of RT-PCR and ELISA, respectively. The expression of p-p85, p85, p-Akt, Akt, p-p65, and p65 were measured by means of Western blotting. RESULTS:
Osthole pre-treatment significantly attenuated renal dysfunction, renal histological changes, NF-κB activation, and the expression of TNF-α, IL-8, and IL-6 induced by I/R injury, but the activation of PI3K/Akt signaling was further increased. CONCLUSIONS:
Osthole pre-treatment protects rats against renal I/R injury by suppressing NF-κB activation, which is involved in PI3K/Akt signaling activation. Thus, osthole may be a novel practical strategy to prevent renal I/R injury.
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Authors | D-Q Xie, G-Y Sun, X-G Zhang, H Gan |
Journal | Transplantation proceedings
(Transplant Proc)
2015 Jul-Aug
Vol. 47
Issue 6
Pg. 1620-6
ISSN: 1873-2623 [Electronic] United States |
PMID | 26293024
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Inc. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents
- Coumarins
- Interleukin-6
- Interleukin-8
- NF-kappa B
- Protective Agents
- Tumor Necrosis Factor-alpha
- Phosphatidylinositol 3-Kinases
- osthol
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Topics |
- Acute Kidney Injury
(etiology, prevention & control)
- Animals
- Anti-Inflammatory Agents
(pharmacology)
- Apoptosis
(drug effects)
- Coumarins
(pharmacology)
- Inflammation
(prevention & control)
- Interleukin-6
(metabolism)
- Interleukin-8
(metabolism)
- Ischemic Preconditioning
(methods)
- Kidney
(blood supply, drug effects)
- Male
- NF-kappa B
(metabolism)
- Oxidative Stress
(drug effects)
- Phosphatidylinositol 3-Kinases
(metabolism)
- Protective Agents
(pharmacology)
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(prevention & control)
- Tumor Necrosis Factor-alpha
(metabolism)
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