Octreotide Protects the Mouse Retina against Ischemic Reperfusion Injury through Regulation of Antioxidation and Activation of NF-κB.

Abstract

Somatostatin (SST), an endogenous peptide, may exert anti-inflammatory and neuroprotective effects on retinal injury induced by ischemia. Retinal ischemic reperfusion (I/R) injury always produces many reactive oxygen species (ROS), which can aggravate the tissue damage. The effects of octreotide (OCT), a SST analogue, on retinal I/R injury and ROS formation, are not very clear. In this study, we observed the effects of OCT on morphological changes, oxidative stress, and cell death, induced by retinal I/R injury. The activation of nuclear factor κB (NF-κB) and intercellular adhesion molecule-1 (ICAM-1) were further evaluated in I/R retina treated with or without OCT. The retinal layer thickness was increased at 1 day after I/R and decreased at 7 days after I/R (P < 0.05). This effect was associated with increase in MDA and ROS levels (P < 0.05). The Tunel-positive cells increased and the number of ganglion cell layer (GCL) neurons decreased significantly after I/R injury. The expression of p-p65 and ICAM-1 increased significantly in I/R retinas (P < 0.05). Each effect was markedly attenuated by application of OCT. These data indicate that OCT protects the retina against retinal I/R damage, which could be through inhibition of oxidative stress and downregulation of NF-κB and ICAM-1 expression.

• Authors: Jun Wang, Ziqiang Sun, Junsheng Shen, Dongdong Wu, Fang Liu, Ruisheng Yang, Shaoping Ji, Ailing Ji, Yanzhang Li
• Journal: Oxidative medicine and cellular longevity (Oxid Med Cell Longev) Vol. 2015 Pg. 970156 ( 2015) ISSN: 1942-0994 [Electronic] United States
• PMID: 26175842 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Protective Agents
• Reactive Oxygen Species
• Transcription Factor RelA
• Intercellular Adhesion Molecule-1
• Malondialdehyde
• Octreotide

Topics

• Animals
• Antioxidants (metabolism)
• Apoptosis (drug effects)
• Cell Survival (drug effects)
• Disease Models, Animal
• Intercellular Adhesion Molecule-1 (genetics, metabolism)
• Malondialdehyde (metabolism)
• Mice
• Mice, Inbred C57BL
• Octreotide (pharmacology, therapeutic use)
• Oxidative Stress (drug effects)
• Protective Agents (pharmacology, therapeutic use)
• Reactive Oxygen Species (metabolism)
• Reperfusion Injury (drug therapy, metabolism, pathology)
• Retina (drug effects, metabolism, pathology)
• Retinal Diseases (drug therapy, metabolism, pathology)
• Transcription Factor RelA (genetics, metabolism)