Coculture with Low-Dose SWCNT Attenuates Bacterial Invasion and Inflammation in Human Enterocyte-like Caco-2 Cells.

Abstract

Single walled carbon nanotubes (SWCNTs) have been shown to be highly effective against a wide range of bacteria. Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) infection is a well-known mediator to prolong hospitalization and initiate chronic inflammation, yet the biological effects of SWCNTs on the pathogen-infected enterocytes remain unclear. Herein, it is shown that the low-dose SWCNT treatment attenuates the human enterocyte-like Caco-2 cells from the damage of E. coli and S. aureus infection by suppressing NLRP3 inflammasome activation. The relatively low-dose (1 and 10 μg mL(-1) ) SWCNT treatments reduce the adhesion and invasion of E. coli and S. aureus to Caco-2 cells, increase the cell viability and proliferation, reduce the tight junction permeability, and restitute the integrity of cell surface microvilli structure, meanwhile has low cytotoxicity to the host cells. The low-dose SWCNT treatment further reduces the NLRP3-mediated IL-1β secretion in the infected cells. The results identify that a low-dose SWCNT treatment serves a protective function for the E. coli- and S. aureus-infected Caco-2 cells by negatively regulating mitochondrial reactive oxygen species-mediated NLRP3 inflammasome activation.

• Authors: Hanqing Chen, Bing Wang, Ruifang Zhao, Di Gao, Ming Guan, Lingna Zheng, Xiaoyan Zhou, Zhifang Chai, Yuliang Zhao, Weiyue Feng
• Journal: Small (Weinheim an der Bergstrasse, Germany) (Small) Vol. 11 Issue 34 Pg. 4366-78 (Sep 09 2015) ISSN: 1613-6829 [Electronic] Germany
• PMID: 26097125 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• CARD Signaling Adaptor Proteins
• Carrier Proteins
• Cytoskeletal Proteins
• Inflammasomes
• Interleukin-1beta
• NLR Family, Pyrin Domain-Containing 3 Protein
• NLRP3 protein, human
• Nanotubes, Carbon
• PYCARD protein, human
• Reactive Oxygen Species
• Superoxides
• Caspase 1

Topics

• Bacterial Adhesion
• CARD Signaling Adaptor Proteins
• Caco-2 Cells
• Carrier Proteins (genetics, metabolism)
• Caspase 1 (genetics, metabolism)
• Cell Shape
• Cell Survival
• Coculture Techniques (methods)
• Cytoskeletal Proteins (genetics, metabolism)
• Enterocytes (microbiology, pathology)
• Epithelium (pathology)
• Escherichia coli (pathogenicity, ultrastructure)
• Humans
• Inflammasomes (metabolism)
• Inflammation (pathology)
• Interleukin-1beta (metabolism)
• Microvilli (ultrastructure)
• Mitochondria (metabolism)
• NLR Family, Pyrin Domain-Containing 3 Protein
• Nanotubes, Carbon (chemistry)
• Reactive Oxygen Species (metabolism)
• Staphylococcus aureus (pathogenicity, ultrastructure)
• Superoxides (metabolism)