[Buyang Huanwu decoction promotes neuroblast migration from subventricular zone via inducing angiogenesis after ischemia].

Abstract

OBJECTIVE:

To study the effect of Buyang Huanwu decoction (BYHWD) inducing angiogenesis on the neuroblast migration from the subventricular zone and its mechanisms after focal cerebral ischemia.

METHOD:

The middle cerebral artery occlusion (MCAO) was performed to mice for 30 minutes to establish the model. The rats were divided into sham group, model group, BYHWD group and endostatin group. BYHWD (20 g x kg(-1), ig) and endostatin (10 μg, sc) were administered 24 h after ischemia once a day for consecutively 14 days. At 14 d after ischemia, the density of micro-vessel and the number of neuroblasts in the ischemia border zone were determined by immunofluorescence staining. The mRNA and protein expression of cell-derived factor-1 (SDF-1) and brain-derived neurotrophic (BDNF) were examined by real-time PCR and Western blot.

RESULT:

Compared with the model group, BYHWD significantly increased the density of micro-vessel and the number of DCX positive cells in the ischemia border zone (P < 0.01), and significantly increased the SDF-1 and BDNF mRNA and protein expression (P < 0.01). Compared with BYHWD group, endostatin significantly reduced the density of micro-vessel and the number of DCX positive cells in the ischemia border zone (P < 0.01), as well as the SDF-1, BDNF mRNA and protein expression (P < 0.01).

CONCLUSION:

BYHWD could promote the neuroblast migration from the subventricular zone via inducing angiogenesis after cerebral ischemia, the mechanism may be correlated with up-regulating the expression of SDF-1 and BDNF.

• Authors: Lin Li, Zhi-ting Liu, Li-sheng Chu, Tian-hong Yu, Tie-bing Qu, Jun Wang, Cui-cui Ren
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 40 Issue 2 Pg. 298-302 (Jan 2015) ISSN: 1001-5302 [Print] China
• PMID: 26080562 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Angiogenesis Inducing Agents
• Brain-Derived Neurotrophic Factor
• Chemokine CXCL12
• Cxcl12 protein, mouse
• Dcx protein, mouse
• Dcx protein, rat
• Doublecortin Protein
• Drugs, Chinese Herbal
• buyang huanwu

Topics

• Angiogenesis Inducing Agents (pharmacology)
• Animals
• Brain Ischemia (pathology, physiopathology)
• Brain-Derived Neurotrophic Factor (analysis, genetics)
• Cell Movement (drug effects)
• Cerebral Ventricles (pathology)
• Chemokine CXCL12 (analysis, genetics)
• Doublecortin Protein
• Drugs, Chinese Herbal (pharmacology)
• Male
• Mice
• Mice, Inbred ICR
• Neurons (drug effects, physiology)