Structural Characterization of Neutral Glycosphingolipids from 3T3-L1 Adipocytes.

Abstract

In recent years, obesity has been considered a pathological stage of early lifestyle-related diseases, and adipose tissue and adipocyte research has been active. Glycosphingolipids are involved in the pathogenesis of type 2 diabetes induced by insulin resistance, but the details of the glycosphingolipid molecular species composition of adipocytes have yet to be elucidated. We used 3T3-L1 adipocytes and the 1,2-dichloroethane-wash method to remove triacylglycerols, which are abundant in adipocytes, and analyzed the structures of glycosphingolipids, particularly neutral glycosphingolipids, using liquid chromatography-mass spectrometry.

• Authors: Hisao Kojima, Yusuke Suzuki, Masahiro Ito, Kazuya Kabayama
• Journal: Lipids (Lipids) Vol. 50 Issue 9 Pg. 913-7 (Sep 2015) ISSN: 1558-9307 [Electronic] United States
• PMID: 26017029 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Neutral Glycosphingolipids

Topics

• 3T3-L1 Cells (chemistry)
• Adipocytes (chemistry)
• Animals
• Cell Line
• Chromatography, Liquid
• Fibroblasts (chemistry)
• Mass Spectrometry
• Mice
• Neutral Glycosphingolipids (chemistry)