Abstract | BACKGROUND: METHODS: RESULTS: The exposure of 20 μM H2O2 for 72 h accelerated E-cadherin loss and vimentin induction in airway epithelial BEAS-2B cells, which was reversed by non-toxic astragalin at 1-20 μM. Astragalin allayed the airway tissue levels of ROS and vimentin enhanced by OVA challenge. Collagen type 1 production increased in H2O2-exposed epithelial cells and collagen fiber deposition was observed in OVA-challenged mouse airways. This study further investigated that the oxidative stress-triggered autophagic regulation was responsible for inducing airway fibrosis. H2O2 highly enhanced the expression induction of the autophagy-related beclin-1 and light chains 3A/B (LC3A/B) within 4 h and astragalin blocked such induction by H2O2. This compound deterred the ROS-promoted autophagosome formation in BEAS-2B cells. Consistently, in OVA-sensitized mice the expression of beclin-1 and LC3A/B was highly induced, and oral administration of astragalin suppressed the autophagosome formation with inhibiting the induction of these proteins in OVA-challenged airway subepithelium. Induction of autophagy by spermidine influenced the epithelial induction of E-cadherin and vimentin that was blocked by treating astragalin. CONCLUSION: These results demonstrate that astragalin can be effective in allaying ROS-promoted bronchial fibrosis through inhibiting autophagosome formation in airways.
|
Authors | In-Hee Cho, Yean-Jung Choi, Ju-Hyun Gong, Daekeun Shin, Min-Kyung Kang, Young-Hee Kang |
Journal | Respiratory research
(Respir Res)
Vol. 16
Pg. 51
(Apr 21 2015)
ISSN: 1465-993X [Electronic] England |
PMID | 25895672
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Apoptosis Regulatory Proteins
- BECN1 protein, human
- Beclin-1
- Cdh1 Proteins
- Collagen Type I
- Fzr1 protein, mouse
- Kaempferols
- Map1lc3b protein, mouse
- Membrane Proteins
- Microtubule-Associated Proteins
- Reactive Oxygen Species
- Vimentin
- Ovalbumin
- astragalin
- Hydrogen Peroxide
- Spermidine
|
Topics |
- Airway Remodeling
(drug effects)
- Animals
- Apoptosis Regulatory Proteins
(metabolism)
- Autophagy
(drug effects)
- Beclin-1
- Cdh1 Proteins
(metabolism)
- Cell Line
- Collagen Type I
(metabolism)
- Cytoprotection
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Epithelial Cells
(drug effects, metabolism, pathology)
- Epithelial-Mesenchymal Transition
(drug effects)
- Humans
- Hydrogen Peroxide
(pharmacology)
- Kaempferols
(pharmacology)
- Lung
(drug effects, pathology)
- Male
- Membrane Proteins
(metabolism)
- Mice, Inbred BALB C
- Microtubule-Associated Proteins
(metabolism)
- Ovalbumin
- Pulmonary Fibrosis
(chemically induced, pathology, prevention & control)
- Reactive Oxygen Species
(metabolism)
- Signal Transduction
(drug effects)
- Spermidine
(pharmacology)
- Time Factors
- Vimentin
(metabolism)
|