Effect of n-3 PUFA supplementation at different EPA:DHA ratios on the spontaneously hypertensive obese rat model of the metabolic syndrome.

Abstract

The increasing incidence of the metabolic syndrome (MetS), a combination of risk factors before the onset of CVD and type 2 diabetes, encourages studies on the role of functional food components such as long-chain n-3 PUFA as preventive agents. In the present study, we explore the effect of EPA and DHA supplementation in different proportions on spontaneously hypertensive obese (SHROB) rats, a model for the MetS in a prediabetic state with mild oxidative stress. SHROB rats were randomised into four groups (n 7), each supplemented with EPA/DHA at ratios of 1:1, 2:1 and 1:2, or soyabean oil as the control for 13 weeks. The results showed that in all the proportions tested, EPA/DHA supplementation significantly lowered total and LDL-cholesterol concentrations, compared with those of the control group. EPA/DHA supplementation at the ratios of 1:1 and 2:1 significantly decreased inflammation (C-reactive protein levels) and lowered oxidative stress (decreased excretion of urinary isoprostanes), mainly at the ratio of 1:2. The activity of antioxidant enzymes increased in erythrocytes, abdominal fat and kidneys, with magnitudes depending on the EPA:DHA ratio. PUFA mixtures from fish affected different MetS markers of CVD risk factors in SHROB rats, depending on the ratios of EPA/DHA supplementation. The activation of endogenous defence systems may be related to the reduction of inflammation and oxidative stress.

• Authors: Eunice Molinar-Toribio, Jara Pérez-Jiménez, Sara Ramos-Romero, Marta Romeu, Montserrat Giralt, Núria Taltavull, Mònica Muñoz-Cortes, Olga Jáuregui, Lucía Méndez, Isabel Medina, Josep Lluís Torres
• Journal: The British journal of nutrition (Br J Nutr) Vol. 113 Issue 6 Pg. 878-87 (Mar 28 2015) ISSN: 1475-2662 [Electronic] England
• PMID: 25720761 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Fish Oils
• Docosahexaenoic Acids
• C-Reactive Protein
• Eicosapentaenoic Acid
• Oxidoreductases

Topics

• Abdominal Fat (enzymology, immunology, metabolism)
• Animals
• Biomarkers (blood, metabolism, urine)
• C-Reactive Protein (analysis)
• Dietary Supplements
• Docosahexaenoic Acids (administration & dosage, therapeutic use)
• Eicosapentaenoic Acid (administration & dosage, therapeutic use)
• Erythrocytes (enzymology, immunology, metabolism)
• Female
• Fish Oils (administration & dosage, therapeutic use)
• Hypercholesterolemia (etiology, prevention & control)
• Hypertension (etiology, prevention & control)
• Kidney (enzymology, immunology, metabolism)
• Metabolic Syndrome (complications, diet therapy, immunology, physiopathology)
• Obesity (complications)
• Oxidative Stress
• Oxidoreductases (blood, metabolism)
• Prediabetic State (etiology, prevention & control)
• Random Allocation
• Rats, Mutant Strains