Confirmation and refinement of the localisation of the c-MEL locus on chromosome 19 by physical and genetic mapping.

Abstract

The human gene locus c-MEL was identified following transfection of genomic DNA from the human melanoma cell line NK14; it has previously been assigned to chromosome 19 (p13.2-q13.2) by analysis of somatic cell hybrids. We have further refined the position of this gene to the proximal region of 19p (cen-p13.2), using cell hybrids containing only fragments of human chromosome 19. We have confirmed this physical localisation by linkage analysis with a recently described restriction fragment length polymorphism for the c-MEL gene, and mapped the locus within the region of the low density lipoprotein receptor gene (LDLR) (Lod 4.43, theta = 0.10) and the anonymous marker D19S11 (13.1.25) (Lod 9.33, theta = 0). This gene thus maps to a region of chromosome 19 involved in karyotypic abnormalities in a variety of malignancies including melanomas and leukaemias.

• Authors: E Nimmo, R A Padua, D Hughes, J D Brook, R Williamson, K J Johnson
• Journal: Human genetics (Hum Genet) Vol. 81 Issue 4 Pg. 382-4 (Mar 1989) ISSN: 0340-6717 [Print] Germany
• PMID: 2564840 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Probes

Topics

• Cell Line
• Chromosome Mapping
• Chromosomes, Human, Pair 19
• DNA Probes
• Genes
• Genetic Linkage
• Humans
• Hybrid Cells
• Polymorphism, Restriction Fragment Length
• Transfection