Abstract | OBJECTIVE: Chronic inflammatory processes are important promotors of temporal lobe epilepsy (TLE) development. Based on human herpesvirus 6 (HHV-6) DNA detection in brain tissue from patients with TLE, an association of persistent viral infection with TLE has been discussed. Individual studies reported increased HHV-6 DNA in patients with clinical signs of previous inflammatory brain reaction, that is, febrile seizures or meningoencephalitis. However, detection rates vary considerably between different studies. Here we performed a large-scale analysis of viral DNA/ RNA spectrum in high-quality TLE biopsies. In addition to all Herpesviridae, we addressed potentially relevant neurotropic RNA viruses. METHODS:
DNA and RNA were extracted from 346 fresh-frozen tissue samples removed by epilepsy surgery. Real-time polymerase chain reaction (PCR) and nested PCR were performed for Herpesviridae and RNA viruses, respectively. Clinical data were analyzed for earlier signs of inflammatory brain reactions. Fresh-frozen hippocampal tissue samples from patients without chronic central nervous system ( CNS) disease served as controls (n = 62). Seven previous PCR studies with overall 178 TLE patients were additionally analyzed regarding a correlation of clinical parameters and HHV-6 detection. RESULTS: PCR revealed HHV-6B DNA in 34 specimens (9.8%) from TLE patients. HHV-6B DNA was also present in eight control samples (12.9%; p > 0.05), but showed a lower virus concentration (p < 0.001). Other herpesviruses and RNA viruses were virtually absent. In patients with clinical signs of previous brain inflammation, HHV-6B DNA was observed in 15.0%, whereas only 6.3% of the samples from patients without febrile seizures or meningoencephalitis were positive for HHV-6B DNA (p < 0.05). A meta-analysis of the eight HHV-6 PCR studies revealed similar results. SIGNIFICANCE: This biopsy-based study shows no differences in frequency of HHV-6B DNA detection between TLE patients and controls. These results do not support the hypothesis of a persistent HHV-6B infection as a major pathogenetic factor in TLE. However, the higher virus load in TLE patients and the increased detection rate of HHV-6B DNA in patients with previous inflammatory brain reactions require further investigations.
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Authors | Laura Esposito, Jan F Drexler, Oliver Braganza, Elke Doberentz, Alexander Grote, Guido Widman, Christian Drosten, Anna M Eis-Hübinger, Susanne Schoch, Christian E Elger, Albert J Becker, Pitt Niehusmann |
Journal | Epilepsia
(Epilepsia)
Vol. 56
Issue 2
Pg. 234-43
(Feb 2015)
ISSN: 1528-1167 [Electronic] United States |
PMID | 25530314
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Wiley Periodicals, Inc. © 2014 International League Against Epilepsy. |
Chemical References |
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Topics |
- Adolescent
- Adult
- Aged
- Biopsy
- DNA, Viral
(analysis)
- Epilepsy, Temporal Lobe
(pathology, virology)
- Female
- Herpesvirus 6, Human
(genetics)
- Humans
- Male
- Middle Aged
- Polymerase Chain Reaction
(methods)
- Young Adult
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