Carcinoembryonic antigen-related cell adhesion molecules (CEACAM) 1, 5 and 6 as biomarkers in pancreatic cancer.

Abstract

BACKGROUND:

Aim of this study was to assess the biological function in tumor progression and metastatic process carcinoembryonic antigen-related cell adhesion molecules (CEACAM) 1, 5 and 6 in pancreatic adenocarcinoma (PDAC).

EXPERIMENTAL DESIGN:

CEACAM knock down cells were established and assessed in vitro and in a subcutaneous and intraperitoneal mouse xenograft model. Tissue and serum expression of patients with PDAC were assessed by immunohistochemistry (IHC) and by enzyme linked immunosorbent assays.

RESULTS:

Presence of lymph node metastasis was correlated with CEACAM 5 and 6 expression (determined by IHC) and tumor recurrence exclusively with CEACAM 6. Patients with CEACAM 5 and 6 expression showed a significantly shortened OS in Kaplan-Meier survival analyses. Elevated CEACAM6 serum values showed a correlation with distant metastasis and. Survival analysis revealed a prolonged OS for patients with low serum CEACAM 1 values. In vitro proliferation and migration capacity was increased in CEACAM knock down PDAC cells, however, mice inoculated with CEACAM knock down cells showed a prolonged overall-survival (OS). The number of spontaneous pulmonary metastasis was increased in the CEACAM knock down group.

CONCLUSION:

The effects mediated by CEACAM expression in PDAC are complex, though overexpression is correlated with loco-regional aggressive tumor growth. However, loss of CEACAM can be considered as a part of epithelial-mesenchymal transition and is therefore of rather importance in the process of distant metastasis.

• Authors: Florian Gebauer, Daniel Wicklein, Jennifer Horst, Philipp Sundermann, Hanna Maar, Thomas Streichert, Michael Tachezy, Jakob R Izbicki, Maximilian Bockhorn, Udo Schumacher
• Journal: PloS one (PLoS One) Vol. 9 Issue 11 Pg. e113023 ( 2014) ISSN: 1932-6203 [Electronic] United States
• PMID: 25409014 (Publication Type: Journal Article)

Chemical References

• Antigens, CD
• Biomarkers, Tumor
• CD66 antigens
• CEACAM5 protein, human
• CEACAM6 protein, human
• Carcinoembryonic Antigen
• Cell Adhesion Molecules
• GPI-Linked Proteins

Topics

• Adult
• Aged
• Aged, 80 and over
• Animals
• Antigens, CD (genetics, metabolism)
• Biomarkers, Tumor (genetics, metabolism)
• Carcinoembryonic Antigen (genetics, metabolism)
• Cell Adhesion Molecules (genetics, metabolism)
• Cell Line, Tumor
• Female
• GPI-Linked Proteins (genetics, metabolism)
• Gene Knockdown Techniques
• Heterografts
• Humans
• Lung Neoplasms (metabolism, secondary)
• Male
• Mice
• Mice, Inbred C57BL
• Middle Aged
• Neoplasm Transplantation
• Pancreatic Neoplasms (metabolism, pathology)
• Survival Analysis