Downregulation of microRNA-429 inhibits cell proliferation by targeting p27Kip1 in human prostate cancer cells.

Abstract

MicroRNAs (miRNAs) are closely associated with cell proliferation, invasion and metastasis in various types of cancer, including prostate cancer. In this study, the role of miR-429 in the regulation of cell proliferation was investigated in prostate cancer cells. miR-429 expression levels were measured in the IF11 and IA8 prostate cancer cell lines and normal prostate epithelial tissues by quantitative polymerase chain reaction. miR-429 mimics or an miR-429 inhibitor were then transfected into the human prostate cancer cell lines. MTT and fluorescence-activated cell sorting were used to detect the effect of miR-429 on cell proliferation. A luciferase reporter system was employed to verify the potential target of miR-429. The results revealed that miR-429 was significantly upregulated in the human prostate cancer cell lines, compared with the normal prostate epithelial tissue. Downregulation of miR-429 expression in IF11 and IA8 cells inhibited cell proliferation and arrested the cells in the G1 phase of the cell cycle. The luciferase assay demonstrated that p27Kip1 was a direct target of miR-429. Furthermore, overexpression of p27Kip1 was observed to partially rescue the proliferation‑promoting effect of miR-429 on IA8 cells. In conclusion, to the best of our knowledge this study was the first to show that miR-429 is involved in the oncogenesis of prostate cancer and thus may be a novel prognostic biomarker in prostate cancer.

• Authors: Yongri Ouyang, Ping Gao, Baoyi Zhu, Xi Chen, Fang Lin, Xi Wang, Junxia Wei, Huizhong Zhang
• Journal: Molecular medicine reports (Mol Med Rep) Vol. 11 Issue 2 Pg. 1435-41 (Feb 2015) ISSN: 1791-3004 [Electronic] Greece
• PMID: 25351256 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 3' Untranslated Regions
• MIRN429 microRNA, human
• MicroRNAs
• RNA, Double-Stranded
• Cyclin-Dependent Kinase Inhibitor p27

Topics

• 3' Untranslated Regions
• Base Sequence
• Binding Sites
• Cell Line, Tumor
• Cell Proliferation (genetics)
• Cyclin-Dependent Kinase Inhibitor p27 (chemistry, genetics, metabolism)
• Down-Regulation
• G1 Phase Cell Cycle Checkpoints
• Humans
• Male
• MicroRNAs (antagonists & inhibitors, genetics, metabolism)
• Prostatic Neoplasms (genetics, pathology)
• RNA, Double-Stranded (metabolism)
• Sequence Alignment
• Transfection
• Up-Regulation