Microarray analysis of gene expression in lung cancer cell lines treated by fractionated irradiation.

Abstract

BACKGROUND:

To identify differentially expressed genes between parent and radioresistant lung cancer cell lines established by fractionated irradiation.

MATERIALS AND METHODS:

Lung cancer cell lines (A549, NCI-H1650) were irradiated with several fractionation schemes. Clonogenic assays were used to identify radioresistant cell lines. We compared the gene expression profiles on a cDNA microarray.

RESULTS:

Four established cell (A549-2G, A549-5G, H1650-2G and H1650-5G) were shown to be radioresistant (p≤0.05). Seventy-two genes were commonly altered in A549-G and 655 genes in H1650-G, compared to their parental cells. Genes in the wingless-type MMTV integration site family (WNT) signaling pathway were the ones most frequently altered in both A549-G and H1650-G cells. Those involved in inflammation; integrin, platelet-derived growth factor (PDGF), interleukin, transforming growth factor-beta (TGFB), epidermal growth factor receptor (EGFR) signaling, were commonly altered in radioresistant H1650 sublines.

CONCLUSION:

The major gene expression changes during irradiation are related to WNT signaling pathway.

• Authors: Sung-Ja Ahn, Chan Choi, Yoo-Duk Choi, Young-Chul Kim, Kyu-Sik Kim, In-Jae Oh, Hee-Jung Ban, Mee-Sun Yoon, Taek-Keun Nam, Jae-Uk Jeong, Joo-Young Song, Woong-Ki Chung
• Journal: Anticancer research (Anticancer Res) Vol. 34 Issue 9 Pg. 4939-48 (Sep 2014) ISSN: 1791-7530 [Electronic] Greece
• PMID: 25202076 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright© 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

Topics

• Cell Line, Tumor
• Cell Proliferation (radiation effects)
• Cell Survival (radiation effects)
• Cluster Analysis
• Dose Fractionation, Radiation
• Dose-Response Relationship, Radiation
• Gene Expression Profiling
• Gene Expression Regulation, Neoplastic (radiation effects)
• Humans
• Lung Neoplasms (genetics, radiotherapy)
• Radiation Tolerance (genetics)
• Reproducibility of Results