Comparison of the effects of CORM-2, CORM-3 and CORM-A1 on coagulation in human plasma.

Abstract

Carbon monoxide derived from the catalytic action of heme oxygenase-1 or carbon monoxide-releasing molecules (CORMs) has been found to potentially be an anticoagulant or procoagulant agent. Of interest, two water-soluble CORMs, CORM-3 and CORM-A1, recently became commercially available. Thus, the purpose of the present study was to assess and compare the effects of the previously well studied CORM-2 to the effects of CORM-3 and CORM-A1 on coagulation in citrated human plasma with thrombelastography. Plasma exposed to CORMs was incubated at 37°C for at least one carbon monoxide release half-time, and then tissue factor-activated coagulation was commenced with calcium addition. CORM-2 and CORM-3 enhanced the velocity of clot formation and thrombus strength in a similar manner, whereas CORM-A1 did not affect coagulation. However, CORM-A1 did diminish tissue-type plasminogen activator initiated fibrinolysis. The similarity in effect on coagulation by CORM-2 and CORM-3 was likely secondary to the relatively inert effect of their ruthenium-containing carrier molecule, whereas the boron-containing CORM-A1 may have had no effect secondary to boron binding to fibrinogen, preventing carbon monoxide-mediated changes in fibrinogen protein structure via attached heme group(s). Future investigations with CORMs should have special attention to confounding effects of the carrier molecule.

• Authors: Vance G Nielsen, Joshua I Garza
• Journal: Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis (Blood Coagul Fibrinolysis) Vol. 25 Issue 8 Pg. 801-5 (Dec 2014) ISSN: 1473-5733 [Electronic] England
• PMID: 25058038 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Boranes
• Carbonates
• Organometallic Compounds
• sodium boranocarbonate
• tricarbonylchloro(glycinato)ruthenium(II)
• tricarbonyldichlororuthenium (II) dimer
• Carbon Monoxide
• Fibrinogen
• Thromboplastin
• Tissue Plasminogen Activator
• Calcium

Topics

• Blood Coagulation
• Boranes (chemistry)
• Calcium (chemistry)
• Carbon Monoxide (chemistry)
• Carbonates (chemistry)
• Fibrinogen (chemistry)
• Humans
• Organometallic Compounds (chemistry)
• Plasma (chemistry)
• Structure-Activity Relationship
• Thrombelastography
• Thromboplastin (chemistry)
• Tissue Plasminogen Activator (chemistry)