Abstract |
AKI is associated with increased morbidity, mortality, and cost of care, and therapeutic options remain limited. Reactive oxygen species are critical for the genesis of ischemic AKI. Stanniocalcin-1 (STC1) suppresses superoxide generation through induction of uncoupling proteins (UCPs), and transgenic overexpression of STC1 inhibits reactive oxygen species and protects from ischemia/reperfusion (I/R) kidney injury. Our observations revealed high AMP-activated protein kinase (AMPK) activity in STC1 transgenic kidneys relative to wild-type (WT) kidneys; thus, we hypothesized that STC1 protects from I/R kidney injury through activation of AMPK. Baseline activity of AMPK in the kidney correlated with the expression of STCs, such that the highest activity was observed in STC1 transgenic mice followed (in decreasing order) by WT, STC1 knockout, and STC1/STC2 double-knockout mice. I/R in WT kidneys increased AMPK activity and the expression of STC1, UCP2, and sirtuin 3. Inhibition of AMPK by administration of compound C before I/R abolished the activation of AMPK, diminished the expression of UCP2 and sirtuin 3, and aggravated kidney injury but did not affect STC1 expression. Treatment of cultured HEK cells with recombinant STC1 activated AMPK and increased the expression of UCP2 and sirtuin 3, and concomitant treatment with compound C abolished these responses. STC1 knockout mice displayed high susceptibility to I/R, whereas pretreatment of STC1 transgenic mice with compound C restored the susceptibility to I/R kidney injury. These data suggest that STC1 is important for activation of AMPK in the kidney, which mediates STC1-induced expression of UCP2 and sirtuin 3 and protection from I/R.
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Authors | Jenny Szu-Chin Pan, Luping Huang, Tatiana Belousova, Lianghao Lu, Yongjie Yang, Roger Reddel, Andy Chang, Huiming Ju, Gabriel DiMattia, Qiang Tong, David Sheikh-Hamad |
Journal | Journal of the American Society of Nephrology : JASN
(J Am Soc Nephrol)
Vol. 26
Issue 2
Pg. 364-78
(Feb 2015)
ISSN: 1533-3450 [Electronic] United States |
PMID | 25012175
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | Copyright © 2015 by the American Society of Nephrology. |
Chemical References |
- Glycoproteins
- Ion Channels
- Mitochondrial Proteins
- Reactive Oxygen Species
- Sirt3 protein, mouse
- Ucp2 protein, mouse
- Uncoupling Protein 2
- Superoxides
- teleocalcin
- Hydrogen Peroxide
- AMP-Activated Protein Kinases
- Sirtuin 3
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Topics |
- AMP-Activated Protein Kinases
(physiology)
- Acute Kidney Injury
(metabolism, physiopathology, prevention & control)
- Animals
- Glycoproteins
(deficiency, genetics, physiology)
- Hydrogen Peroxide
(metabolism)
- Ion Channels
(metabolism)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Mice, Transgenic
- Mitochondrial Proteins
(metabolism)
- Models, Animal
- Reactive Oxygen Species
(metabolism)
- Reperfusion Injury
(metabolism, physiopathology, prevention & control)
- Signal Transduction
(physiology)
- Sirtuin 3
(metabolism)
- Superoxides
(metabolism)
- Uncoupling Protein 2
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