[Up-regulation of the CXCR4 expression by hypoxia in maxillary sinus carcinoma cells IMC3].

Abstract

OBJECTIVE:

To investigate the expression of CXCR4 in maxillary sinus carcinoma cells IMC3 under hypoxia.

METHOD:

IMC3 cells were cultured for 6 h, 12 h, 24 h and 48 h under normoxia and hypoxia. Real-Time PCR was applied to detect the expression of mRNA of CXCR4 and immunohistochemisrty was applied to investigate its protein level.

RESULT:

CXCR4 mRNA level was about 0.035 under normal conditions, which was obviously upregulated by hypoxia. The mRNA levels after culturing under hypoxia for 6 h, 12 h, 24 h and 48 h were 0.283, 0.313, 0.426, 0.510 respectively. There was statistically significant difference between the mRNA levels of each two groups (P < 0.05, Mann-Whiney Test) with a time dependent course, except for the difference between the groups of 6 h and 12 h. Immunohistochemistry showed that there was almost negative staining for CXCR4 in the cell cultured in nomoxia, while stong positive staining of CXCR4 was observed in cells cultured in hypoxia . The positive staining was located mainly in the cell membrane and cytoplasm and little in the nucleus.

CONCLUSION:

Hypoxia could induce expression of CXCR4 in IMC3 cells at both mRNA and ptrotein levels. The upregulation of CXCR4 by hypoxia showed an obvious time dependent course.

• Authors: Lizhong Su, Zhiming Zhang, Wenyue Ji
• Journal: Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery (Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi) Vol. 28 Issue 8 Pg. 548-50 (Apr 2014) ISSN: 2096-7993 [Print] China
• PMID: 25007671 (Publication Type: English Abstract, Journal Article)

Chemical References

• CXCR4 protein, human
• Receptors, CXCR4

Topics

• Cell Hypoxia
• Cell Line, Tumor
• Gene Expression Regulation, Neoplastic
• Humans
• Maxillary Sinus (metabolism, pathology)
• Receptors, CXCR4 (metabolism)