Abstract | BACKGROUND: METHODS: MCF7 cells were treated with BfL. Viability and adhesion alterations were evaluated using flow cytometry and western blotting. RESULTS:
BfL inhibited the viability of the MCF7 cell line but was ineffective on MDA-MB-231 and MCF 10A cells. It inhibits MCF7 adhesion on laminin, collagen I and fibronectin, decreases α1, α6 and β1 integrin subunit expression, and increases α5 subunit expression. BfL triggers necrosis and secondary necrosis, with caspase-9 inhibition. It also causes deoxyribonucleic acid (DNA) fragmentation, which leads to cell cycle arrest in the G2/M phase and a decrease in the expression of the regulatory proteins pRb and p21. CONCLUSION:
BfL shows selective cytotoxic effect and adhesion inhibition on MCF7 breast cancer cells. GENERAL SIGNIFICANCE: Cell death induction and inhibition of cell adhesion may contribute to understanding the action of lectins in breast cancer.
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Authors | Mariana C C Silva, Cláudia A A de Paula, Joana G Ferreira, Edgar J Paredes-Gamero, Angela M S F Vaz, Misako U Sampaio, Maria Tereza S Correia, Maria Luiza V Oliva |
Journal | Biochimica et biophysica acta
(Biochim Biophys Acta)
Vol. 1840
Issue 7
Pg. 2262-71
(Jul 2014)
ISSN: 0006-3002 [Print] Netherlands |
PMID | 24641823
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier B.V. All rights reserved. |
Chemical References |
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Topics |
- Bauhinia
(chemistry)
- Breast Neoplasms
(drug therapy, pathology)
- Cell Adhesion
(drug effects)
- Cell Death
(drug effects)
- Cell Survival
(drug effects)
- Female
- Humans
- Integrins
(metabolism)
- Lectins
(chemistry, pharmacology)
- MCF-7 Cells
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