Abstract | PURPOSE: METHODS: The lung carcinoma samples were collected from 113 patients diagnosed as NSCLC at Tumor Hospital of Xiangya School of Medicine from January 2005 to December 2008. Immunohistochemistry was performed for TLR5 and the protein expression score was quantified using an established scoring system. Kaplan-Meier survival curves were generated for disease-free survival (DFS) and overall survival (OS) in all patients. TLR5 expression levels were correlated with DFS and OS using univariate and multivariate Cox regression analysis. Expression and subcellular localization of TLR5 were analyzed in NSCLC cell lines including SPC-A1, A549, H1975, and H1299 cells. Activation of TLR5 signaling pathway by flagellin was characterized by western blotting. Effects of flagellin on NSCLC cell proliferation, anchorage-independent growth, migration, and invasion were analyzed by BrdU incorporation assay, soft agar colony formation assay, wound-healing migration assay, and transwell invasion assay, respectively. RESULTS: High expression of TLR5 was significantly associated with better prognosis in patients with NSCLC. We further demonstrated that activation of TLR5 by flagellin in NSCLC cells inhibited cell proliferation, migration, and invasion in vitro. CONCLUSION: TLR5 may serve as a potential therapeutic target in NSCLC patients.
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Authors | Hui Zhou, Jian-hua Chen, Jun Hu, Yong-zhong Luo, Fang Li, Ling Xiao, Mei-zuo Zhong |
Journal | Journal of cancer research and clinical oncology
(J Cancer Res Clin Oncol)
Vol. 140
Issue 4
Pg. 633-43
(Apr 2014)
ISSN: 1432-1335 [Electronic] Germany |
PMID | 24549739
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- RNA, Messenger
- TLR5 protein, human
- Toll-Like Receptor 5
- Luciferases
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Topics |
- Adenocarcinoma
(diagnosis, metabolism, pathology)
- Biomarkers, Tumor
(metabolism)
- Blotting, Western
- Carcinoma, Non-Small-Cell Lung
(diagnosis, metabolism, pathology)
- Carcinoma, Squamous Cell
(diagnosis, metabolism, pathology)
- Cell Movement
- Cell Proliferation
- Enzyme-Linked Immunosorbent Assay
- Female
- Follow-Up Studies
- Humans
- Immunoenzyme Techniques
- Luciferases
(metabolism)
- Lung Neoplasms
(diagnosis, metabolism, pathology)
- Male
- Middle Aged
- Neoplasm Staging
- Prognosis
- RNA, Messenger
(genetics)
- Survival Rate
- Toll-Like Receptor 5
(metabolism)
- Wound Healing
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