Hydroxycinnamic acids in Crepidiastrum denticulatum protect oxidative stress-induced retinal damage.

Abstract

We investigated the effects of an ethanol extract of C. denticulatum (EECD) in a mouse model of glaucoma established by optic nerve crush (ONC), and found that EECD significantly protected against retinal ganglion cell (RGC) death caused by ONC. Furthermore, EECD effectively protected against N-methyl-d-aspartate-induced damage to the rat retinas. In vitro, EECD attenuated transformed retinal ganglion cell (RGC-5) death and significantly blunted the up-regulation of apoptotic proteins and mRNA level induced by 1-buthionine-(S,R)-sulfoximine combined with glutamate, reduced reactive oxygen species production by radical species, and inhibited lipid peroxidation. The major EECD components were found to be chicoric acid and 3,5-dicaffeoylquinic acid (3,5-DCQA) that have shown beneficial effects on retinal degeneration both in vitro and in vivo studies. Thus, EECD could be used as a natural neuroprotective agent for glaucoma, and chicoric acid and 3,5-DCQA as mark compounds for the development of functional food.

• Authors: Hong Ryul Ahn, Hee Ju Lee, Kyung-A Kim, Chul Young Kim, Chu Won Nho, Holim Jang, Cheol-Ho Pan, Chang Yong Lee, Sang Hoon Jung
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 62 Issue 6 Pg. 1310-23 (Feb 12 2014) ISSN: 1520-5118 [Electronic] United States
• PMID: 24428171 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Caffeic Acids
• Coumaric Acids
• Neuroprotective Agents
• Plant Extracts
• Succinates
• Chlorogenic Acid
• N-Methylaspartate
• 3,5-dicaffeoylquinic acid
• chicoric acid

Topics

• Animals
• Apoptosis (drug effects)
• Asteraceae (chemistry)
• Caffeic Acids (administration & dosage, analysis)
• Chlorogenic Acid (administration & dosage, analogs & derivatives, analysis)
• Coumaric Acids (administration & dosage, analysis)
• Disease Models, Animal
• Glaucoma (etiology, prevention & control)
• Lipid Peroxidation (drug effects)
• Male
• Mice
• Mice, Inbred ICR
• N-Methylaspartate (administration & dosage)
• Nerve Crush
• Neuroprotective Agents
• Optic Nerve
• Oxidative Stress
• Plant Extracts (administration & dosage)
• Rats
• Rats, Sprague-Dawley
• Retinal Diseases (etiology, prevention & control)
• Succinates (administration & dosage, analysis)