Neuropilin-1 is overexpressed in osteosarcoma and contributes to tumor progression and poor prognosis.

Abstract

AIM:

Neuropilin (NRP)-1, a co-receptor for vascular endothelial growth factor (VEGF), plays an important role in angiogenesis and malignant progression of many cancers. However, the involvement of NRP-1 in osteosarcoma is not completely understood. The aim of this study was to investigate the expression pattern and clinical significance of NRP-1 in human osteosarcoma.

METHODS:

NRP-1 mRNA and protein expression levels were detected by RT-PCR and Western blot assays, respectively, using 166 pairs of osteosarcoma and noncancerous bone tissues. Then, the association of NRP-1 expression with clinicopathological factors or survival of osteosarcoma patients was further evaluated.

RESULTS:

RT-PCR and Western blot assays revealed that NRP-1 expression in osteosarcoma tissues was significantly higher than that in corresponding noncancerous bone tissues at both mRNA and protein levels (both P < 0.001). In addition, high NRP-1 expression more frequently occurred in osteosarcoma tissues with advanced clinical stage (P = 0.006), positive distant metastasis (P = 0.01) and poor response to chemotherapy (P = 0.006). Moreover, osteosarcoma patients with high NRP-1 expression had significantly shorter overall survival and disease-free survival (both P < 0.001) when compared with patients with the low expression of NRP-1. On Cox multivariate analysis, NRP-1 overexpression was an independent and significant prognostic factor to predict poor overall survival and disease-free survival (both P = 0.001).

CONCLUSION:

This is the first study to reveal that NRP-1 overexpression may be related to the prediction of metastasis potency, response to chemotherapy and poor prognosis for osteosarcoma patients, suggesting that NRP-1 may serve as a prognostic marker for the optimization of clinical treatments.

• Authors: H Zhu, H Cai, M Tang, J Tang
• Journal: Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico (Clin Transl Oncol) Vol. 16 Issue 8 Pg. 732-8 (Aug 2014) ISSN: 1699-3055 [Electronic] Italy
• PMID: 24338507 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Neuropilin-1

Topics

• Adult
• Aged
• Biomarkers, Tumor (analysis)
• Blotting, Western
• Bone Neoplasms (mortality, pathology)
• Disease Progression
• Disease-Free Survival
• Female
• Humans
• Kaplan-Meier Estimate
• Male
• Middle Aged
• Neuropilin-1 (biosynthesis)
• Osteosarcoma (mortality, pathology)
• Prognosis
• Proportional Hazards Models
• Real-Time Polymerase Chain Reaction
• Reverse Transcriptase Polymerase Chain Reaction