Upregulation of the long non-coding RNA PlncRNA-1 promotes esophageal squamous carcinoma cell proliferation and correlates with advanced clinical stage.

Abstract

BACKGROUND:

Recent studies revealed that long noncoding RNAs (lncRNAs) play critical regulatory roles in cancer biology. PlncRNA-1 is one of lncRNAs that is associated with cell apoptosis and proliferation of prostate cancer.

AIM:

This study aimed to assess the potential role of PlncRNA-1 in the pathogenesis of esophageal squamous cell carcinoma (ESCC).

MATERIALS AND METHODS:

Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression level of PlncRNA-1 in 73 pairs of ESCC and their matched normal tissues. The correlation of PlncRNA-1 with clinicopathological features and clinical stages was also analyzed. Cancer cell proliferation and apoptosis were assessed following knock-down of PlncRNA-1 by MTT, colony formation assay, and flow cytometry.

RESULTS:

The expression of PlncRNA-1 was significantly higher in human ESCC compared with the adjacent noncancerous tissues (69.8 %, p < 0.05), and the high level of PlncRNA-1 expression was significantly correlated with advanced clinical stage (p < 0.01) and lymph node metastasis (p < 0.05). Furthermore, knockdown of PlncRNA-1 reduced cell proliferation and increased the apoptosis in vitro.

CONCLUSIONS:

PlncRNA-1 plays an important role in ESCC cell proliferation. Overexpression of PlncRNA-1 is correlated with advanced tumor stage and lymph node metastasis, and may serve as a potential prognostic marker and therapeutic target for ESCC.

• Authors: Chun-Mei Wang, Qing-Quan Wu, Su-Qing Li, Fang-Jun Chen, Lei Tuo, Hai-Wei Xie, Yu-Suo Tong, Lv Ji, Guo-Zhi Zhou, Gang Cao, Ming Wu, Jin Lv, Wei-Hong Shi, Xiu-Feng Cao
• Journal: Digestive diseases and sciences (Dig Dis Sci) Vol. 59 Issue 3 Pg. 591-7 (Mar 2014) ISSN: 1573-2568 [Electronic] United States
• PMID: 24337686 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• PlncRNA-1, human
• RNA, Long Noncoding

Topics

• Adult
• Aged
• Aged, 80 and over
• Apoptosis
• Biomarkers, Tumor (metabolism)
• Carcinoma, Squamous Cell (genetics, metabolism, pathology)
• Cell Line, Tumor
• Cell Proliferation
• Esophageal Neoplasms (genetics, metabolism, pathology)
• Female
• Flow Cytometry
• Gene Expression Regulation, Neoplastic
• Gene Knockdown Techniques
• Humans
• Male
• Middle Aged
• Neoplasm Staging
• RNA, Long Noncoding (metabolism)
• Real-Time Polymerase Chain Reaction
• Reverse Transcriptase Polymerase Chain Reaction
• Up-Regulation