Abstract | ETHNOPHARMACOLOGICAL RELEVANCE:
Moutan Cortex (MC, family: Paeonia suffruticosa Andr.) is a well-known traditional herbal medicine that has been shown to hold a protective effect on inflammation in several diseases. However, its anti-inflammatory activity on diabetic nephropathy (DN) has been less reported. The present study was conducted to evaluate the potential attenuation activities of MC on inflammation in AGEs-induced rat mesangial cells dysfunction and high- glucose-fat diet and streptozotocin (STZ)-induced DN rats and explore the possible mechanism underlying its DN effect. MATERIALS AND METHODS: RESULTS: Pretreatment with MC could significantly inhibit AGEs-induced migration of macrophages in the co-culture system of mesangial cell and macrophage. MC could decrease IL-6 and MCP-1 levels in serum of DN rats in a dose-dependent manner. Furthermore, MC also improved the blood glucose, serum creatinine and urine protein levels. Both immunocytochemistry analysis and western blot analysis showed that MC decreased significantly the over-expression of IL-6, MCP-1, TGF-β1, ICAM-1 and RAGE in mesangial cells or kidney tissues. Additionally, the protein expression of proinflammatory cytokine could also be down-regulated by the pretreatment of RAGE-Ab (5 μg/ml). CONCLUSION: These findings indicated that the extract of MC had an amelioration activity on the inflammation in AGEs-induced mesangial cells dysfunction and high- glucose-fat diet and STZ-induced DN rats. The protective effect might be associated with the intervention of MC via target of RAGE. These findings suggested that MC might be a benefit agent for the prevention and treatment of DN.
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Authors | Ming-hua Zhang, Liang Feng, Mao-mao Zhu, Jun-fei Gu, Jun Jiang, Xu-dong Cheng, Shu-ming Ding, Chan Wu, Xiao-bin Jia |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 151
Issue 1
Pg. 591-600
( 2014)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 24269777
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2013 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents
- Dietary Fats
- Drugs, Chinese Herbal
- Glycation End Products, Advanced
- moutan cortex
- Glucose
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Topics |
- Animals
- Anti-Inflammatory Agents
(chemistry, pharmacology)
- Cell Line
- Diabetes Mellitus, Experimental
(complications)
- Diabetic Nephropathies
(drug therapy)
- Dietary Fats
(adverse effects)
- Dose-Response Relationship, Drug
- Drugs, Chinese Herbal
(chemistry, pharmacology)
- Gene Expression Regulation
(drug effects)
- Glucose
(administration & dosage, adverse effects)
- Glycation End Products, Advanced
(toxicity)
- Mesangial Cells
(drug effects, metabolism)
- Paeonia
(chemistry)
- Random Allocation
- Rats
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