Abstract | UNLABELLED: METHODOLOGY/PRINCIPAL FINDINGS: We used the proportional bactericidal method. 210 four-week-old immunodeficient female Nude mice (NMRI-Foxn1(nu) /Foxn1(nu) ) were inoculated in the left hind footpad with 0.03 ml of bacterial suspension containing 5 × 10(3), 5 × 10(2), 5 × 10(1), and 5 × 10(0) M. leprae AFB organisms of strain Hoshizuka-4 which is a multidrug resistant strain harboring a GyrA A91V substitution. An additional subgroup of 10 mice was inoculated with 5 × 10(-1) bacilli in the untreated control group. The day after inoculation, subgroups of mice were treated with a single dose of ofloxacin, moxifloxacin, garenoxacin or clarithromycin at 150 mg/kg dosing. 12 months later mice were sacrificed and M. leprae bacilli were numbered in the footpad. The results from the untreated control group indicated that the infective inoculum contained 23% of viable M. leprae. The results from the moxifloxacin and garenoxacin groups indicated that a single dose of these drugs reduced the percentage of viable M. leprae by 90%, similarly to the reduction observed after a single dose of the positive control drug clarithromycin. Conversely, ofloxacin was less active than clarithromycin. CONCLUSION/SIGNIFICANCE:
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Authors | Nicolas Veziris, Aurélie Chauffour, Sylvie Escolano, Sarah Henquet, Masanori Matsuoka, Vincent Jarlier, Alexandra Aubry |
Journal | PLoS neglected tropical diseases
(PLoS Negl Trop Dis)
Vol. 7
Issue 11
Pg. e2559
(Nov 2013)
ISSN: 1935-2735 [Electronic] United States |
PMID | 24244784
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Fluoroquinolones
- Quinolines
- Quinolones
- quinoline
- Moxifloxacin
- garenoxacin
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Topics |
- Animals
- Drug Resistance, Multiple, Bacterial
- Female
- Fluoroquinolones
(therapeutic use)
- Leprosy
(drug therapy, microbiology)
- Mice
- Moxifloxacin
- Mycobacterium leprae
(drug effects, pathogenicity)
- Quinolines
(therapeutic use)
- Quinolones
(therapeutic use)
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