Histopathological correlations of islet amyloidosis with apolipoprotein E polymorphisms in type 2 diabetic Chinese patients.

Abstract

OBJECTIVE:

Islet amyloidosis and arteriosclerosis are histopathological hallmarks in type 2 diabetes. Apolipoprotein E (ApoE) is a common component of amyloidosis. ApoE [Latin Small Letter Open E]4 allele is associated with arteriosclerosis and cerebral amyloidosis in Alzheimer disease. We examined the correlations of ApoE polymorphisms with islet amyloidosis in type 2 diabetes.

METHODS:

Genomic DNA samples were obtained from 117 autopsy cases with type 2 diabetes and 209 nondiabetic cases. ApoE genotypes and amylin gene mutations were determined by polymerase chain reaction-ligase detection reaction analysis. Islet amyloidosis and arteriosclerosis were evaluated by staining of thioflavin T, amylin, ApoE, and amyloid P component.

RESULTS:

In the diabetic group, 33.3% in group [Latin Small Letter Open E]2 ([Latin Small Letter Open E]2[Latin Small Letter Open E]2, [Latin Small Letter Open E]2[Latin Small Letter Open E]3), 23.6% in group [Latin Small Letter Open E]3 ([Latin Small Letter Open E]3[Latin Small Letter Open E]3), and 62.5% in group [Latin Small Letter Open E]4 ([Latin Small Letter Open E]4[Latin Small Letter Open E]4, [Latin Small Letter Open E]3[Latin Small Letter Open E]4) had islet amyloidosis. After adjustment for confounders, group [Latin Small Letter Open E]4 had an odds ratio of 7.0 (95% confidence interval, 1.3-38.0; P = 0.023) in having islet amyloidosis compared to group [Latin Small Letter Open E]3. Diabetic cases with islet amyloidosis had more severe arteriosclerosis (P = 0.0111), arteriolar hyalinosis (P = 0.0369), and interstitial fibrosis (P = 0.0188) than those without amyloidosis. Immunoreactivity of both ApoE and amyloid P component was detected in islet amyloid deposits and arteriosclerotic lesions.

CONCLUSIONS:

In type 2 diabetes, islet amyloidosis and arteriosclerosis share common pathophysiological features with ApoE [Latin Small Letter Open E]4 as a probable linking factor.

• Authors: Jing Guan, Hai-Lu Zhao, Yi Sui, Lan He, Heung-Man Lee, Fernand M M Lai, Peter C Y Tong, Juliana C N Chan
• Journal: Pancreas (Pancreas) Vol. 42 Issue 7 Pg. 1129-37 (Oct 2013) ISSN: 1536-4828 [Electronic] United States
• PMID: 24005233 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Apolipoproteins E
• Islet Amyloid Polypeptide
• Serum Amyloid P-Component

Topics

• Aged
• Aged, 80 and over
• Amyloidosis (complications, genetics, pathology)
• Apolipoproteins E (genetics, metabolism)
• Arteriosclerosis (complications, genetics, pathology)
• Asian People (genetics)
• Case-Control Studies
• China
• Diabetes Mellitus, Type 2 (complications, genetics, pathology)
• Female
• Humans
• Islet Amyloid Polypeptide (genetics)
• Islets of Langerhans (pathology)
• Male
• Middle Aged
• Pancreatic Diseases (complications, genetics, pathology)
• Polymorphism, Genetic
• Serum Amyloid P-Component (metabolism)