Pulsed-focused ultrasound enhances boron drug accumulation in a human head and neck cancer xenograft-bearing mouse model.

Abstract

PURPOSE:

This study aims to demonstrate that pulsed high-intensity focused ultrasound (pulsed-HIFU) may enhance the fructose-conjugated 4-borono-L-phenylalanine (BPA-Fr) accumulation in tumor lesion using (18)F-FBPA-Fr microPET scans.

PROCEDURES:

To the mice bearing orthotopic SASC03 human tongue squamous carcinoma xenograft, a 2-min pulsed-HIFU was applied to tumor. Immediately after pulsed-HIFU treatment, (18)F-FBPA-Fr was intravenously injected, and biological characterizations including microPET imaging and biodistribution were conducted.

RESULTS:

Both biodistribution studies and microPET imaging performed after intravenous injection of (18)F-FBPA-Fr revealed higher tumor uptake in HIFU-treated mice than that of the control. CD31 and Ki-67 histochemical staining of tumor sections and H&E staining of nearby normal tissues revealed no significant difference between the pulsed-HIFU-treated mice and the control.

CONCLUSION:

This study demonstrated that pulsed-HIFU was beneficial to the accumulation of boron drug in the head and neck tumor lesion and may enhance the therapeutic efficacy of clinical BNCT.

• Authors: Chun-Yi Wu, Pei-Chia Chan, Lin-Shan Chou, Chi-Wei Chang, Feng-Yi Yang, Ren-Shyan Liu, Shih-Hwa Chiou, Yi-Wei Chen, Sang-Hue Yen, Hsin-Ell Wang
• Journal: Molecular imaging and biology (Mol Imaging Biol) Vol. 16 Issue 1 Pg. 95-101 (Feb 2014) ISSN: 1860-2002 [Electronic] United States
• PMID: 23925592 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 4-borono-2-fluorophenylalaninefructose
• Boron Compounds
• Glycopeptides

Topics

• Animals
• Area Under Curve
• Boron Compounds (pharmacology)
• Carcinoma, Squamous Cell (diagnostic imaging, pathology)
• Cell Line, Tumor
• Glycopeptides (pharmacology)
• Head and Neck Neoplasms (diagnostic imaging, pathology)
• High-Intensity Focused Ultrasound Ablation
• Humans
• Mice
• Positron-Emission Tomography
• Tissue Distribution (drug effects)
• Tomography, X-Ray Computed
• Tongue Neoplasms (diagnostic imaging, pathology)
• Xenograft Model Antitumor Assays