Neutrophil-infiltrated paw edema induced by mannose-binding Dioclea violacea lectin.

Abstract

BACKGROUND:

The potential edematogenic effect and the pharmacological characterization of a glucose-mannose-binding lectin from Dioclea violacea (DvL) were investigated.

METHODS:

Paw edema was induced with DvL in control animals, and in animals pretreated with glucocorticoid or with blockers of histamine, nitric oxide synthase, cyclooxygenase, platelet activating factor (PAF), bradykinin and lipoxygenase.

RESULTS:

DvL-induced paw edema paralleled with an increase in vascular permeability and myeloperoxidase (MPO) activity. DvL-induced edema could be prevented by pre-treatment with the lectin-binding sugar α-D-methyl mannoside. Dexamethasone, meclizine and Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME) inhibited this effect.

CONCLUSIONS:

DvL induces edema, increase in vascular permeability and neutrophil infiltration. The edematogenic activity involves the lectin mannose-binding sites and is associated with histamine, cytokines and nitric oxide, since it could be treated with meclizine, dexamethasone and L-NAME.

• Authors: Nylane Maria N de Alencar, Mário Rogério L Mota, Natália V F C Rodrigues, Jorge Luiz Martins, Kyria S do Nascimento, Ana Maria S Assreuy, Benildo S Cavada
• Journal: Pharmacological reports : PR (Pharmacol Rep) Vol. 65 Issue 1 Pg. 220-5 ( 2013) ISSN: 2299-5684 [Electronic] Switzerland
• PMID: 23563042 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Mannose-Binding Lectin
• Nitric Oxide
• Meclizine
• Dexamethasone
• Histamine
• Peroxidase
• NG-Nitroarginine Methyl Ester

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Binding Sites
• Cytokines (metabolism)
• Dexamethasone (pharmacology)
• Dioclea (chemistry)
• Disease Models, Animal
• Edema (chemically induced, prevention & control)
• Female
• Histamine (metabolism)
• Mannose-Binding Lectin (isolation & purification, toxicity)
• Meclizine (pharmacology)
• NG-Nitroarginine Methyl Ester (pharmacology)
• Neutrophil Infiltration (drug effects)
• Neutrophils (metabolism)
• Nitric Oxide (metabolism)
• Peroxidase (metabolism)
• Rats
• Rats, Wistar