SoSoSo or its active ingredient chrysophanol regulates production of inflammatory cytokines & adipokine in both macrophages & adipocytes.

Abstract

BACKGROUND & OBJECTIVES:

Obesity is now considered as a major risk factor for the development of fatty liver diseases, cardiovascular diseases, and atherosclerosis. SoSoSo is a newly developed dietary supplement made of seven medicinal herbs. This study was aimed at examining the anti-obesity effect of SoSoSo or its active ingredient chrysophanol on the production of inflammatory cytokines and adipokine in macrophyage cell line RAW264 and 3T3-L1 adipocytes.

METHODS:

No release was measured as a form of nitrite by Griess method. The production of inflammatory cytokines and adipokine were measured with the ELISA method. The m-RNA expression of each cytokine and adipokine were measured using RT-PCR. The nuclear proteins for NF-κB were analyzed with western blotting.

RESULTS:

SoSoSo or chrysophanol significantly inhibited the nitric oxide production in lipopolysaccharide-stimulated RAW264 cells as well as in RAW264 cells-conditioned medium (CM)-treated 3T3-L1 cells. The production of interleukin (IL)-6 and tumour necrosis factor (TNF)-α were inhibited by SoSoSo or chrysophanol. In addition, SoSoSo or chrysophanol inhibited the activation of nuclear factor-κB in RAW264 cells. SoSoSo or chrysophanol inhibited the productions of IL-6, TNF-α, and monocyte chemoattractant protein-1 as well as the reduction of adiponectin production in CM-treated 3T3-L1 cells.

INTERPRETATION & CONCLUSIONS:

These results suggest a potential of SoSoSo or chrysophanol as a source of anti-inflammatory agent for obesity. Further in vivo studies would be required to confirm these findings.

• Authors: Hong-Kun Rim, Phil-Dong Moon, In-Hwa Choi, Eun-Hee Lee, Hyung-Min Kim, Hyun-Ja Jeong
• Journal: The Indian journal of medical research (Indian J Med Res) Vol. 137 Issue 1 Pg. 142-50 (Jan 2013) ISSN: 0971-5916 [Print] India
• PMID: 23481064 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adipokines
• Anthraquinones
• Cytokines
• NF-kappa B
• chrysophanic acid

Topics

• 3T3-L1 Cells
• Adipocytes (drug effects, metabolism)
• Adipokines (biosynthesis)
• Animals
• Anthraquinones (administration & dosage, chemistry)
• Cytokines (biosynthesis)
• Dietary Supplements
• Gene Expression Regulation
• Humans
• Macrophages (drug effects)
• Mice
• NF-kappa B (biosynthesis)
• Obesity (diet therapy, genetics, pathology)
• Plants, Medicinal