Abstract | BACKGROUND: METHODS: A microarray and qRT-PCR were performed to investigate the miRNA expression profiles in PC-3 sphere cells and adherent cells. A transwell assay was used to evaluate the migration of PC-3 sphere cells and adherent cells. MiR-143 was silenced with antisense oligonucleotides in PC-3, PC-3-M and LNCaP cells. The role of miR-143 in prostate cancer metastasis was measured by wound-healing and transwell assays in vitro and bioluminescence imaging in vivo. Bioinformatics and luciferase report assays were used to identify the target of miR-143. RESULTS: The expression of miR-143 and the migration capability were reduced in PC-3 sphere cells and progressively increased during sphere re-adherent culture. Moreover, the down-regulation of miR-143 suppressed prostate cancer cells migration and invasion in vitro and systemically inhibited metastasis in vivo. Fibronectin type III domain containing 3B (FNDC3B), which regulates cell motility, was identified as a target of miR-143. The inhibition of miR-143 increased the expression of FNDC3B protein but not FNDC3B mRNA in vitro and vivo. CONCLUSIONS: These data demonstrate for the first time that miR-143 was up-regulated during the differentiation of prostate cancer stem cells and promoted prostate cancer metastasis by repressing FNDC3B expression. This sheds a new insight into the post-transcriptional regulation of cancer stem cells differentiation by miRNAs, a potential approach for the treatment of prostate cancer.
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Authors | Xinlan Fan, Xu Chen, Weixi Deng, Guangzheng Zhong, Qingqing Cai, Tianxin Lin |
Journal | BMC cancer
(BMC Cancer)
Vol. 13
Pg. 61
(Feb 05 2013)
ISSN: 1471-2407 [Electronic] England |
PMID | 23383988
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- FNDC3B protein, human
- Fibronectins
- MIRN143 microRNA, human
- MicroRNAs
- Neoplasm Proteins
- RNA, Neoplasm
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Topics |
- Cell Differentiation
(physiology)
- Cell Line, Tumor
- Cell Movement
(physiology)
- Fibronectins
(metabolism)
- Humans
- Male
- MicroRNAs
(physiology)
- Microarray Analysis
- Neoplasm Proteins
(metabolism)
- Neoplastic Stem Cells
(physiology)
- Prostatic Neoplasms
(metabolism, pathology)
- RNA, Neoplasm
(physiology)
- Reverse Transcriptase Polymerase Chain Reaction
- Up-Regulation
- Wound Healing
(physiology)
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