Acute macrovascular dysfunction in patients with type 2 diabetes induced by ingestion of advanced glycated β-lactoglobulins.

Abstract

OBJECTIVE:

Recent evidence indicates that heat-enhanced food advanced glycation end products (AGEs) adversely affect vascular function. The aim of this study was to examine the acute effects of an oral load of heat-treated, AGE-modified β-lactoglobulins (AGE-BLG) compared with heat-treated, nonglycated BLG (C-BLG) on vascular function in patients with type 2 diabetes mellitus (T2DM).

RESEARCH DESIGN AND METHODS:

In a double-blind, controlled, randomized, crossover study, 19 patients with T2DM received, on two different occasions, beverages containing either AGE-BLG or C-BLG. We measured macrovascular [brachial ultrasound of flow-mediated dilatation (FMD)] and microvascular (laser-Doppler measurements of reactive hyperemia in the hand) functions at baseline (T0), 90 (T90), and 180 (T180) min.

RESULTS:

Following the AGE-BLG, FMD decreased at T90 by 80% from baseline and remained decreased by 42% at T180 (P < 0.05 vs. baseline, P < 0.05 vs. C-BLG at T90). By comparison, following C-BLG, FMD decreased by 27% at T90 and 51% at T180 (P < 0.05 vs. baseline at T180). A significant decrease in nitrite (T180) and nitrate (T90 and T180), as well as a significant increase in N(ε)-carboxymethyllisine, accompanied intake of AGE-BLG. There was no change in microvascular function caused by either beverage.

CONCLUSIONS:

In patients with T2DM, acute oral administration of a single AGE-modified protein class significantly though transiently impaired macrovascular function in concert with decreased nitric oxide bioavailability. These AGE-related changes were independent of heat treatment.

• Authors: Alin Stirban, Paraskevi Kotsi, Knut Franke, Ulf Strijowski, Weijing Cai, Christian Götting, Diethelm Tschoepe
• Journal: Diabetes care (Diabetes Care) Vol. 36 Issue 5 Pg. 1278-82 (May 2013) ISSN: 1935-5548 [Electronic] United States
• PMID: 23238657 (Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)

Chemical References

• Glycation End Products, Advanced
• Lactoglobulins

Topics

• Adult
• Aged
• Cross-Over Studies
• Diabetes Mellitus, Type 2 (metabolism, physiopathology)
• Female
• Glycation End Products, Advanced (adverse effects)
• Humans
• Lactoglobulins (adverse effects)
• Male
• Microcirculation (drug effects)
• Middle Aged