Apoptosis-inducing and antitumor activity of neolignans isolated from Magnolia officinalis in HeLa cancer cells.

Abstract

Two neolignans, 4'-methoxymagndialdehyde (1) and magnaldehyde B (2), were isolated from the stem bark of Magnolia officinalis (Magnoliaceae), evaluated for apoptosis-inducing effects in human cervical epitheloid carcinoma HeLa cells. The apoptosis-inducing activity of compounds 1 and 2 were assessed by DNA content using flow cytometric analysis. In the immunoblotting analysis, the treatment with 1 and 2 resulted in the cleavage of procaspase-8 and -3 and poly(ADP-ribose)polymerase into active forms. In addition, in vivo, the administration of 2 to Lewis lung carcinoma-inoculated mice evidenced a significant inhibition of tumor growth (volume) with reduction of 28.7% at concentration of 20 mg/kg, as compared with the control mice. These findings suggest that 2 can inhibit the proliferation of tumor cells, and might be an anti-tumoric agent.

• Authors: Ui Joung Youn, Nighat Fatima, Quan Cheng Chen, Sungwook Chae, Tran Manh Hung, Byung-Sun Min
• Journal: Phytotherapy research : PTR (Phytother Res) Vol. 27 Issue 9 Pg. 1419-22 (Sep 2013) ISSN: 1099-1573 [Electronic] England
• PMID: 23192855 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 John Wiley & Sons, Ltd.

Chemical References

• 4'-methoxymagndialdehyde
• Antineoplastic Agents, Phytogenic
• Lignans
• magnaldehyde B
• Poly(ADP-ribose) Polymerases
• CASP3 protein, human
• CASP8 protein, human
• Caspase 3
• Caspase 8

Topics

• Animals
• Antineoplastic Agents, Phytogenic (pharmacology)
• Apoptosis (drug effects)
• Carcinoma, Lewis Lung
• Caspase 3 (metabolism)
• Caspase 8 (metabolism)
• Cell Proliferation (drug effects)
• HeLa Cells
• Humans
• Lignans (pharmacology)
• Magnolia (chemistry)
• Male
• Mice
• Plant Bark (chemistry)
• Poly(ADP-ribose) Polymerases (metabolism)