Abstract | BACKGROUND: METHODS: miR-122, miR-141, miR-155, miR-184, miR-200c, miR-210, miR-224, and miR-514, validated as differentially expressed in a previous study, were measured by RT-PCR in matched malignant and non-malignant tumor samples after nephrectomy from 111 patients (89 without, 22 with metastases) and clinicopathological and outcome data were collected. Non-parametric statistical tests, receiver-operating characteristics, Kaplan-Meier-, and univariate as well as multivariate Cox regression analyses were performed. RESULTS: Downregulation of miR-141/-184/-200c/-514 and upregulation of miR-122/-155/-210/-224 were not different between samples of non-metastatic and metastatic tumors except for miR-122 and miR-514. miR-514 was further downregulated in metastatic compared with non-metastatic tumors while the upregulation of miR-122 was significantly reduced in metastatic carcinomas. All miRNAs were suitable to discriminate malignant from non-malignant tissue. miR-122 and miR-514 were significantly related to the recurrence risk but only miR-514 provided independent prognostic information in the final model including relevant clinicopathological variables. CONCLUSIONS: MiR-122 and miR-514 play a role in tumor recurrence after nephrectomy. Expression of miR-514 was particularly downregulated in primary metastatic tumor and those that recur and might be a suitable adjunct marker for predicting tumor recurrence.
|
Authors | Zofia Wotschofsky, Jonas Busch, Monika Jung, Carsten Kempkensteffen, Steffen Weikert, Klaus D Schaser, Ingo Melcher, Ergin Kilic, Kurt Miller, Glen Kristiansen, Andreas Erbersdobler, Klaus Jung |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 416
Pg. 5-10
(Feb 01 2013)
ISSN: 1873-3492 [Electronic] Netherlands |
PMID | 23178446
(Publication Type: Clinical Trial, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2012 Elsevier B.V. All rights reserved. |
Chemical References |
- Biomarkers, Tumor
- MicroRNAs
|
Topics |
- Adult
- Aged
- Aged, 80 and over
- Biomarkers, Tumor
- Carcinoma, Renal Cell
(diagnosis, physiopathology, surgery)
- Down-Regulation
- Female
- Gene Expression Profiling
- Humans
- Male
- MicroRNAs
(metabolism)
- Middle Aged
- Neoplasm Metastasis
- Nephrectomy
- Polymerase Chain Reaction
- Prognosis
- Regression Analysis
|