Although
phosphite is widely used to protect plants from pathogenic oomycetes on a wide range of horticultural crops, the molecular mechanisms behind
phosphite induced resistance are poorly understood. The aim of this work was to assess the effects of
potassium phosphite (KPhi) on potato plant defense responses to
infection with Phytophtora infestans (Pi). Pathogen development was severely restricted and there was also an important decrease in lesion size in infected KPhi-treated leaves. We demonstrated that KPhi primed
hydrogen peroxide and
superoxide anion production in potato leaves at 12 h post-inoculation with Pi. Moreover, the KPhi-treated leaves showed an increased and earlier
callose deposition as compared with water-treated plants, beginning 48 h after inoculation. In contrast,
callose deposition was not detected in water-treated leaves until 72 h after inoculation. In addition, we carried out
RNA gel blot analysis of genes implicated in the responses mediated by salicylic (SA) and
jasmonic acid (JA). To this end, we examined the temporal expression pattern of StNPR1 and StWRKY1, two
transcription factors related to SA pathway, and StPR1 and StIPII, marker genes related to SA and JA pathways, respectively. The expression of StNPR1 and StWRKY1 was enhanced in response to KPhi treatment. In contrast, StIPII was down regulated in both KPhi- and water-treated leaves, until 48 h after
infection with Pi, suggesting that the regulation of this gene could be independent of the KPhi treatment. Our results indicate that KPhi primes the plant for an earlier and more intense response to
infection and that SA would mediate this response.