[Expression of monocarboxylate transporter 8 mRNA in the brain tissue of rats with cerebral ischemia].

Abstract

OBJECTIVE:

To investigate the mRNA expression of monocarboxylate transporter 8 (MCT8), a thyroid hormone transport protein, in the lateral ventricle of rats with cerebral ischemia.

METHODS:

Immunofluorescence staining was used to observe the expression of MCT8 in the lateral ventricle of 5 normal SD rats. Another 20 adult male SD rats were randomized into 4 groups and subject to permanent ligation of both the common carotid arteries (2-vessel occlusion, 2VO) for 3 days, 2 weeks, or 5 weeks, or no ligation (control). At the end of the experiment, the transcriptional level of MCT8 in the brain tissue of the rats were detected using fluorescent quantitative PCR.

RESULTS:

MCT8 mRNA levels in 3-day and 2-week 2VO groups were comparable with that in the control group (P=0.909; P=0.694), but increased significantly in 5-week 2VO group compared with that in the control and 3-day 2VO groups (P=0.029; P=0.023). No significance was found in MCT8 mRNA between the 2-week and 5-week 2VO groups (P=0.065).

CONCLUSION:

Prolonged cerebral ischemia causes compensatory increase of MCT8 mRNA expression on the capillary endothelial cell membranes in the lateral ventricle of rats.

• Authors: Dan Cui, Yuqing Guan, Haishan Jang, Jin Wang, Lei Xi, Qun Wang
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 32 Issue 6 Pg. 913-5 (Jun 2012) ISSN: 1673-4254 [Print] China
• PMID: 22699085 (Publication Type: English Abstract, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Monocarboxylic Acid Transporters
• monocarboxylate transporter 8, rat

Topics

• Animals
• Brain (metabolism)
• Brain Ischemia (metabolism)
• Cerebral Infarction (metabolism)
• Male
• Monocarboxylic Acid Transporters (metabolism)
• Rats
• Rats, Sprague-Dawley