Association between apolipoprotein E polymorphism and subclinic atherosclerosis in patients with type 1 diabetes mellitus.

Abstract

OBJECTIVE:

The most important cause of morbidity and mortality in type 1 diabetes mellitus (DM) is atherosclerosis. Apolipoprotein E (Apo E) polymorphism is accused of being the genetic risk factor for atherosclerosis. The aim of the present study was to determine which Apo E polymorphism was related to atherosclerosis in patients with type 1 DM.

METHODS:

Seventy-four patients with type 1 DM were enrolled in the study. Age, diabetes duration, daily insulin dose, microalbuminuria, and major cardiovascular risk factors including anthropometric and metabolic parameters were assessed in each patient. Non-invasive ultrasonographic measurements were also performed. For determination of Apo E genotype, DNA was extracted from venous blood from all subjects using standard methods. Apo E genotyping was performed using a PCR-restriction fragment-length polymorphism assay.

RESULTS:

Systolic blood pressure and carotid artery intima-media thickness (CA-IMT) were increased in subjects with E4/E4 polymorphism. According to univariate analysis, when adjusted for all risk factors, genotypes did not differ for total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and triglycerides (p>0.05). However, E3/E3, E3/E4 and E4/E4 genotypes were found to be associated with an increase in CA-IMT (p<0.001).

CONCLUSIONS:

Our results suggest that the polymorphism associated with atherosclerosis in type1 DM is Apo E4/E4.

• Authors: Mehmet Emre Atabek, Yusuf Özkul, Beray Selver Eklioğlu, Selim Kurtoğlu, Murat Baykara
• Journal: Journal of clinical research in pediatric endocrinology (J Clin Res Pediatr Endocrinol) Vol. 4 Issue 1 Pg. 8-13 (Mar 2012) ISSN: 1308-5735 [Electronic] Turkey
• PMID: 22394699 (Publication Type: Journal Article)

Chemical References

• Apolipoproteins E
• Cholesterol, HDL
• Cholesterol, LDL
• Triglycerides
• Cholesterol

Topics

• Adolescent
• Apolipoproteins E (genetics)
• Atherosclerosis (complications, genetics)
• Blood Pressure
• Carotid Intima-Media Thickness
• Child
• Cholesterol (blood)
• Cholesterol, HDL (blood)
• Cholesterol, LDL (blood)
• Diabetes Mellitus, Type 1 (blood, complications, physiopathology)
• Female
• Genetic Predisposition to Disease (genetics)
• Genotype
• Humans
• Linear Models
• Male
• Multivariate Analysis
• Polymerase Chain Reaction
• Polymorphism, Genetic
• Polymorphism, Restriction Fragment Length
• Risk Factors
• Triglycerides (blood)