Expression and prognostic relevance of PRAME in primary osteosarcoma.

Abstract

The preferentially expressed antigen of melanoma (PRAME), a cancer-testis antigen with unknown function, is expressed in many human malignancies and is considered an attractive potential target for tumor immunotherapy. However, studies of its expression and function in osteosarcoma have rarely been reported. In this study, we found that PRAME is expressed in five osteosarcoma cell lines and in more than 70% of osteosarcoma patient specimens. In addition, an immunohistochemical analysis showed that high PRAME expression was associated with poor prognosis and lung metastasis. Furthermore, PRAME siRNA knockdown significantly suppressed the proliferation, colony formation, and G1 cell cycle arrest in U-2OS cells. Our results suggest that PRAME plays an important role in cell proliferation and disease progression in osteosarcoma. However, the detail mechanisms of PRAME function in osteosarcoma require further investigation.

• Authors: Pingxian Tan, Changye Zou, Bicheng Yong, Ju Han, Longjuan Zhang, Qiao Su, Junqiang Yin, Jin Wang, Gang Huang, Tingsheng Peng, Jingnian Shen
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 419 Issue 4 Pg. 801-8 (Mar 23 2012) ISSN: 1090-2104 [Electronic] United States
• PMID: 22390931 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Crown Copyright © 2012. Published by Elsevier Inc. All rights reserved.

Chemical References

• Antigens, Neoplasm
• PRAME protein, human
• RNA, Small Interfering

Topics

• Antigens, Neoplasm (biosynthesis, genetics)
• Bone Neoplasms (metabolism, pathology)
• Cell Line, Tumor
• Cell Proliferation
• Gene Knockdown Techniques
• Humans
• Osteosarcoma (metabolism, pathology)
• Prognosis
• RNA, Small Interfering (genetics)
• Transfection