Anti-inflammatory effects of Tat-Annexin protein on ovalbumin-induced airway inflammation in a mouse model of asthma.

Abstract

Chronic airway inflammation is a key feature of bronchial asthma. Annexin-1 (ANX1) is an anti-inflammatory protein that is an important modulator and plays a key role in inflammation. Although the precise action of ANX1 remains unclear, it has emerged as a potential drug target for inflammatory diseases such as asthma. To examine the protective effects of ANX1 protein on ovalbumin (OVA)-induced asthma in animal models, we used a cell-permeable Tat-ANX1 protein. Mice sensitized and challenged with OVA antigen had an increased amount of cytokines and eosinophils in their bronchoalveolar lavage (BAL) fluid. However, administration of Tat-ANX1 protein before OVA challenge significantly decreased the levels of cytokines (interleukin (IL)-4, IL-5, and IL-13) and BAL fluid in lung tissues. Furthermore, OVA significantly increased the activation of mitogen-activated protein kinase (MAPK) in lung tissues, whereas Tat-ANX1 protein markedly reduced phosphorylation of MAPKs such as extracellular signal-regulated protein kinase, p38, and stress-activated protein kinase/c-Jun N-terminal kinase. These results suggest that transduced Tat-ANX1 protein may be a potential protein therapeutic agent for the treatment of lung disorders including asthma.

• Authors: Sun Hwa Lee, Dae Won Kim, Hye Ri Kim, Su Jung Woo, So Mi Kim, Hyo Sang Jo, Seong Gyu Jeon, Sung-Woo Cho, Jong Hoon Park, Moo Ho Won, Jinseu Park, Won Sik Eum, Soo Young Choi
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 417 Issue 3 Pg. 1024-9 (Jan 20 2012) ISSN: 1090-2104 [Electronic] United States
• PMID: 22222376 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Inc. All rights reserved.

Chemical References

• Annexin A1
• Annexins
• Anti-Inflammatory Agents, Non-Steroidal
• Cytokines
• Gene Products, tat
• Recombinant Fusion Proteins
• Tat-ANX1 fusion protein
• Ovalbumin

Topics

• Animals
• Annexin A1 (administration & dosage, therapeutic use)
• Annexins (administration & dosage, therapeutic use)
• Anti-Inflammatory Agents, Non-Steroidal (administration & dosage, therapeutic use)
• Asthma (drug therapy, prevention & control)
• Cytokines (antagonists & inhibitors, biosynthesis)
• Disease Models, Animal
• Female
• Gene Products, tat (administration & dosage, therapeutic use)
• Mice
• Mice, Inbred BALB C
• Ovalbumin
• Recombinant Fusion Proteins (administration & dosage, therapeutic use)