Abstract | BACKGROUND:
Transforming growth factor-α (TGF-α) has been shown to augment mesenchymal stem cell-mediated cardioprotection during acute ischemia and reperfusion in isolated heart models. To determine whether this pretreatment strategy would be effective in vivo, we hypothesized that the intramyocardial injection of mesenchymal stem cells pretreated with TGF-α after coronary artery ligation would confer greater preservation of cardiac function, reduction in infarct size, and reduction myocardial inflammation. METHODS: Sprague-Dawley rats underwent left anterior descending coronary artery ligation. Ischemic border zones were injected 30 minutes later with vehicle (n = 11), 1 million mesenchymal stem cells (n = 9), or mesenchymal stem cells pretreated with TGF-α (250 ng/mL for 24 hours; n = 10). Cardiac function was assessed by echocardiography at 7 and 28 days after ligation. Infarct size was measured using triphenyltetrazolium chloride. Ischemic border zone cytokine expression was measured 30 days after infarction. RESULTS: CONCLUSIONS: Intramyocardial injection of mesenchymal stem cells pretreated with TGF-α further protects cardiac function and reduces infarct size compared with injection of untreated cells. Pretreating donor cells with TGF-α may be useful for enhancing cell-based therapies for myocardial ischemia.
|
Authors | Jeremy L Herrmann, Aaron M Abarbanell, Yue Wang, Brent R Weil, Jeffrey A Poynter, Mariuxi C Manukyan, Daniel R Meldrum |
Journal | The Annals of thoracic surgery
(Ann Thorac Surg)
Vol. 92
Issue 5
Pg. 1719-25
(Nov 2011)
ISSN: 1552-6259 [Electronic] Netherlands |
PMID | 21944441
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
|
Copyright | Copyright © 2011 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Transforming Growth Factor alpha
|
Topics |
- Animals
- Combined Modality Therapy
- Male
- Mesenchymal Stem Cell Transplantation
- Myocardial Ischemia
(prevention & control)
- Rats
- Rats, Sprague-Dawley
- Transforming Growth Factor alpha
(therapeutic use)
|